Pandey P C, Upadhyay S, Upadhyay B
Department of Chemistry, Banaras Hindu University, Varanasi, India.
Anal Biochem. 1997 Oct 1;252(1):136-42. doi: 10.1006/abio.1997.2291.
This article describes the research investigations on the development of the amperometric biosensors based on mediated bioelectrochemistry. The mediated bioelectrochemistry involving horseradish peroxidase and glucose oxidase within the graphite paste is reported. The enzyme horseradish peroxidase together with electrochemical mediator was incorporated within the graphite paste electrode. The amperometric response is based on the mediated electrochemical regeneration of peroxidase within the paste. The mediated electrochemical regeneration of peroxidase and glucose oxidase was studied and compared using three different electron transfer mediators-tetracyanoquinodimethane (TCNQ), tetrathiafulvalene (TTF), and dimethyl ferrocene (dmFc). The mediated electrochemistry involving these three mediators was studied based on the cyclic voltammetry. The electrochemical measurements show that TTF is better mediator for the development of peroxide biosensor compared to TCNQ and dmFc. On the other hand, TCNQ is better mediator for the development of glucose sensor. The response curves for peroxide have been reported using these three mediators. A comparative study on the amperometric response based on the mediated electrochemical oxidation of peroxidase and glucose oxidase has also been made using these three mediators. The various parameters, i.e., background current, stability of the mediator within the graphite paste during the electrochemical measurements, and reproducibility of the amperometric response, are discussed.
本文描述了基于介导生物电化学的安培型生物传感器开发的研究调查。报道了在石墨糊中涉及辣根过氧化物酶和葡萄糖氧化酶的介导生物电化学。辣根过氧化物酶与电化学媒介体一起被纳入石墨糊电极中。安培响应基于糊剂中过氧化物酶的介导电化学再生。使用三种不同的电子转移媒介体——四氰基对苯二醌二甲烷（TCNQ）、四硫富瓦烯（TTF）和二甲基二茂铁（dmFc），研究并比较了过氧化物酶和葡萄糖氧化酶的介导电化学再生。基于循环伏安法研究了涉及这三种媒介体的介导电化学。电化学测量表明，与TCNQ和dmFc相比，TTF是开发过氧化物生物传感器的更好媒介体。另一方面，TCNQ是开发葡萄糖传感器的更好媒介体。已报道了使用这三种媒介体的过氧化物响应曲线。还使用这三种媒介体对基于过氧化物酶和葡萄糖氧化酶的介导电化学氧化的安培响应进行了比较研究。讨论了各种参数，即背景电流、电化学测量期间石墨糊中媒介体的稳定性以及安培响应的重现性。
