Nagasawa A, Kubota R, Imamura Y, Nagamine K, Wang Y, Asakawa S, Kudoh J, Minoshima S, Mashima Y, Oguchi Y, Shimizu N
Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan.
Genomics. 1997 Sep 15;44(3):273-9. doi: 10.1006/geno.1997.4889.
We isolated cDNAs for a novel protein with a calculated molecular mass of 46 kDa, containing a leucine-rich repeat (LRR) with conserved flanking sequences and a C2-type immunoglobulin (Ig)-like domain. This novel protein was considered to be a new member of the Ig superfamily and was named ISLR (immunoglobulin superfamily containing LRR). These domains are known to be important for protein-protein interaction or cell adhesion, and therefore it is possible that the novel protein ISLR may also interact with other proteins or cells. Northern blot analysis showed the presence of a 2.4-kb transcript in various human tissues including retina, heart, skeletal muscle, prostate, ovary, small intestine, thyroid, adrenal cortex, testis, stomach, and spinal cord as well as fetal lung and fetal kidney. The ISLR gene was mapped on human chromosome 15q23-q24 by fluorescence in situ hybridization.
我们分离出了一种新型蛋白质的cDNA，其计算分子量为46 kDa，包含一个具有保守侧翼序列的富含亮氨酸重复序列（LRR）和一个C2型免疫球蛋白（Ig）样结构域。这种新型蛋白质被认为是Ig超家族的新成员，并被命名为ISLR（包含LRR的免疫球蛋白超家族）。已知这些结构域对于蛋白质-蛋白质相互作用或细胞黏附很重要，因此新型蛋白质ISLR也有可能与其他蛋白质或细胞相互作用。Northern印迹分析显示，在包括视网膜、心脏、骨骼肌、前列腺、卵巢、小肠、甲状腺、肾上腺皮质、睾丸、胃和脊髓以及胎儿肺和胎儿肾在内的各种人体组织中存在一个2.4 kb的转录本。通过荧光原位杂交将ISLR基因定位到人类染色体15q23 - q24上。
