Saupe S, Roizès G, Peter M, Boyle S, Gardiner K, De Sario A
Séquences Répétées et Centromères Humains, CNRS UPR 1142, Institut de Biologie, 4, Boulevard Henri IV, Montpellier, 34060, France.
Genomics. 1998 Sep 15;52(3):305-11. doi: 10.1006/geno.1998.5439.
We report the isolation and characterization of a novel cDNA IGSF3 that contains a 3648-bp open reading frame encoding an apparent immunoglobulin (Ig)-like membrane protein characterized by eight Ig domains. IGSF3 has an overall structural similarity and strong sequence similarity to V7, a human leukocyte surface protein. The IGSF3 mRNA is highly expressed in placenta, kidney, and lung, but is also present in a wide range of other human tissues. Although a small internal sequence of the IGSF3 cDNA hybridizes to a YAC derived from the centromeric region of chromosome 21, in situ hybridization experiments on human metaphase chromosomes show that the gene corresponding to the long cDNA that we cloned is located in chromosome band 1p13 and that related sequences are located on chromosomes 2 and 13. These data serve to emphasize the potential difficulties in transcriptional mapping in centromeric regions.
我们报告了一种新型cDNA IGSF3的分离和特性，该cDNA包含一个3648 bp的开放阅读框，编码一种明显的免疫球蛋白（Ig）样膜蛋白，其特征为有八个Ig结构域。IGSF3与人类白细胞表面蛋白V7在整体结构上相似，且序列相似度很高。IGSF3 mRNA在胎盘、肾脏和肺中高度表达，但在广泛的其他人体组织中也有存在。尽管IGSF3 cDNA的一小段内部序列与来自21号染色体着丝粒区域的一个酵母人工染色体（YAC）杂交，但对人类中期染色体进行的原位杂交实验表明，我们克隆的长cDNA对应的基因位于1p13染色体带，相关序列位于2号和13号染色体上。这些数据突显了在着丝粒区域进行转录图谱绘制时可能遇到的困难。
