Fujiwara T, Kobayashi T, Takaya J, Taniuchi S, Kobayashi Y
Department of Pediatrics, Kansai Medical University, Osaka, Japan.
Clin Immunol Immunopathol. 1997 Oct;85(1):67-72. doi: 10.1006/clin.1997.4403.
To elucidate the defense mechanism in neonates against bacterial infections, phagocytic activity and hydrogen peroxide (H2O2) production by polymorphonuclear leukocytes (PMNs) in the whole blood and the effects of plasma on these functions were investigated on 44 healthy mature neonates (term 37 to 41 weeks) and 15 premature neonates (term 30 to 36 weeks) using two color flow cytometric analysis. The results were compared to those of a healthy adult control group (n = 10). PMN phagocytic activity was low in both mature and premature neonates. H2O2 production of PMN with phorbol myristate acetate (PMA) stimulation and following phagocytosis was augmented in both mature and premature neonates. When plasma and PMNs of adults and neonates were separated and combined differently, phagocytic activity and H2O2 production of PMNs appeared to be principally regulated by the plasma employed. This finding indicates that plasma has major effects on both phagocytosis and H2O2 production by PMNs of newborn neonates.
为阐明新生儿抵御细菌感染的防御机制，采用双色流式细胞术分析，对44例健康足月新生儿（胎龄37至41周）和15例早产新生儿（胎龄30至36周）的全血中多形核白细胞（PMN）的吞噬活性和过氧化氢（H2O2）生成情况以及血浆对这些功能的影响进行了研究。将结果与健康成人对照组（n = 10）的结果进行比较。足月和早产新生儿的PMN吞噬活性均较低。在足月和早产新生儿中，用佛波酯（PMA）刺激并吞噬后，PMN的H2O2生成均增加。当将成人和新生儿的血浆与PMN分离并以不同方式组合时，PMN的吞噬活性和H2O2生成似乎主要受所用血浆的调节。这一发现表明，血浆对新生新生儿PMN的吞噬作用和H2O2生成均有主要影响。
