Tsan M F, Douglass K H, McIntyre P A
Blood. 1977 Mar;49(3):437-44.
The effects of bacterial neuraminidase on production of hydrogen peroxide (H2O2) and killing of Staphylococcus aureus by human polymorphonuclear leukocytes (PMN) were studied. The concentration of H2O2 was measured by the disappearance of scopoletin fluorescence in the presence of horseradish peroxidase. The results indicated that desialylation of human PMN inhibited the stimulation of H2O2 production during phagocytosis. It also markedly impaired the killing of S. aureus. Impaired killing of S. aureus by desialylated PMN was due to impaired intracellular killing rather than defective phagocytosis.
研究了细菌神经氨酸酶对人多形核白细胞（PMN）产生过氧化氢（H2O2）及杀灭金黄色葡萄球菌的影响。在辣根过氧化物酶存在的情况下，通过 scopoletin 荧光消失来测定 H2O2 的浓度。结果表明，人 PMN 的去唾液酸化抑制了吞噬作用期间 H2O2 的产生刺激。它还显著损害了对金黄色葡萄球菌的杀灭。去唾液酸化的 PMN 对金黄色葡萄球菌的杀灭受损是由于细胞内杀灭受损而非吞噬作用缺陷。
