Evans C R, Pierrepoint C G
J Endocrinol. 1976 Jul;70(1):31-7. doi: 10.1677/joe.0.0700031.
Nuclei have been isolated from canine prostatic tissue previously incubated with tritiated testosterone or tritiated 5alpha-androstane-3alpha, 17alpha-diol. The protein-bound radioactivity has, in both cases, been shown to be associated with 5alpha-androstane-3alpha, 17alpha-diol. The formation and translocation of this steroid to the nucleus supports previous evidence that it has an active part to play in prostatic function in the dog. The formation from testosterone of another titriated steroid, possibly 5alpha-dihydrotestosterone, was also demonstrated although this product did not enter the nucleus and was apparently bound to the nuclear membrane.
已从先前用氚标记的睾酮或氚标记的5α-雄甾烷-3α，17α-二醇孵育过的犬前列腺组织中分离出细胞核。在这两种情况下，与蛋白质结合的放射性均已显示与5α-雄甾烷-3α，17α-二醇相关。这种类固醇在细胞核中的形成和转运支持了先前的证据，即它在犬的前列腺功能中发挥着积极作用。还证实了从睾酮形成另一种氚标记的类固醇，可能是5α-二氢睾酮，尽管该产物未进入细胞核，显然与核膜结合。
