Djoseland O, Hastings C D, Hansson V
Steroids. 1976 Nov;28(5):585-96. doi: 10.1016/0039-128x(76)90001-5.
The pattern of androgenic metabolites in blood, muscle, caput and cauda epididymidis has been investigated in functionally hepatectomized 24 hours castrated rats, 3 hours after the intra-muscular injection of 200 muCi of 3H-3H-3alpha-diol. Identification of the radioactive metabolites showed only negligible differences between the epididymal regions. In both caput and cauda the main metabolite was DHT (17beta-hydroxy-5alpha-androstane-3-one); 3alpha- and 3beta-diol, androsterone (3alpha-hydroxy-5alpha-androstane-17-one), 5-A-dione (5alpha-androsterone-3, 17-dione), delta16-3alpha-01 (kalpha-androst-16-en-3alpha-01), delta16-3beta-01 (5alpha-androst-16-en-3alpha-01) and delta16-3-one (5alpha-androst-16-en-3-one) were also present. Androsterone and 3alpha-diol were the predominant metabolites in blood and muscle. No delta16 compounds could be detected and in constrast to epididymis, more than 50% of the radioactivity was associated with polar compounds. From determination of total radioactivity, it was seen that retention by epididymis varied from two to four times that of muscle. Purification and identification of the radioactivity associated with the nuclear fraction demonstrated that DHT was the only nuclear bound androgen. It is suggested from these results that at least one effect of 3alpha-diol on the rat epididymis is exerted through its conversion to DHT.
在功能性肝切除24小时后阉割的大鼠中，于肌肉注射200微居里的3H-3H-3α-二醇3小时后，研究了血液、肌肉、附睾头和附睾尾中雄激素代谢物的模式。放射性代谢物的鉴定显示附睾区域之间只有微不足道的差异。在附睾头和附睾尾中，主要代谢物都是双氢睾酮（17β-羟基-5α-雄甾烷-3-酮）；还存在3α-和3β-二醇、雄酮（3α-羟基-5α-雄甾烷-17-酮）、5-A-二酮（5α-雄甾酮-3,17-二酮）、δ16-3α-醇（5α-雄甾-16-烯-3α-醇）、δ16-3β-醇（5α-雄甾-16-烯-3β-醇）和δ-十六-3-酮（5α-雄甾-16-烯-3-酮）。雄酮和3α-二醇是血液和肌肉中的主要代谢物。未检测到δ16化合物，与附睾相反，超过50%的放射性与极性化合物相关。从总放射性的测定来看，附睾的保留量是肌肉的两到四倍。与核部分相关的放射性的纯化和鉴定表明双氢睾酮是唯一与核结合的雄激素。从这些结果表明，3α-二醇对大鼠附睾的至少一种作用是通过其转化为双氢睾酮来发挥的。
