D'Eustachio P, Cohen J E, Edelman G M
J Exp Med. 1976 Jul 1;144(1):259-65. doi: 10.1084/jem.144.1.259.
To determine the extent and nature of individual variation in the development of specific antigen-binding cells, the numbers of cells specific for each of two antigens in the spleens of individual random-bred Swiss-L and inbred CBA/J and BALB/c fetal mice were measured as a function of spleen size. For Swiss-L fetuses, the ratio of antigen-binding cells to nucleateated cells varied more than would arise from sampling fluctuation. For each inbred strain, however, the number of cells specific for a given antigen was a constant proportion of the total number of nucleated cells within sampling error. These proportions varied from antigen to antigen, and from strain to strain. The ratio of the proportions of cells specific for the two antigens, however, differed no more from CBA/J to BALB/c mice than would be expected in repeated samples of cells from the spleen of a single fetus. These results confirm at the level of the individual fetus the uniform pattern of development seen for populations of fetuses. They reveal a surprising precision in the proliferation of specific antigen-binding cell populations and suggest that the development of these cells may be subject to strong genetic controls.
为了确定特定抗原结合细胞发育过程中个体差异的程度和性质，我们测量了随机繁殖的瑞士- L以及近交系CBA/J和BALB/c胎鼠脾脏中针对两种抗原中每种抗原的细胞数量，并将其作为脾脏大小的函数。对于瑞士- L胎鼠，抗原结合细胞与有核细胞的比例变化比抽样波动引起的变化更大。然而，对于每个近交系，在抽样误差范围内，针对给定抗原的细胞数量是有核细胞总数的恒定比例。这些比例因抗原和品系而异。然而，两种抗原特异性细胞比例的比值在CBA/J和BALB/c小鼠之间的差异，并不比从单个胎鼠脾脏中重复抽样细胞所预期的差异更大。这些结果在个体胎鼠水平上证实了在胎鼠群体中观察到的一致发育模式。它们揭示了特定抗原结合细胞群体增殖中惊人的精确性，并表明这些细胞的发育可能受到强大的遗传控制。
