Schön R
J Clin Chem Clin Biochem. 1976 Mar;14(3):159-60.
With slight practical modification, especially in the preparation of blood samples on filter paper in place of liquid blood samples, it was possible to adapt the determination of galactose dehydrogenase (Weidemann, G. (1971), this Journal 9,527) to mass screening. The technique is described, and the first results are briefly discussed and compared with those obtained over the years in our screening program with proven the Guthrie test (1): The dehydrogenase test is much quicker and shows a higher substrate specificity than the Guthrie test (duration 2 hours compared with 44 hours; false positive results of 16.87 mmol/l galactose or higher 1:42231 compared with 1:3519). It is, however, less sensitive (16.87 mmol/l compared with 1.69 mmol/l galactose), so that a combination of both tests would seem appropriate for the mass screening of new borns.
经过一些实际的改进，特别是用滤纸制备血样代替液态血样，就可以使半乳糖脱氢酶的测定方法（魏德曼，G.（1971年），本刊9卷，527页）适用于大规模筛查。本文描述了该技术，并简要讨论了首批结果，并与多年来我们在筛查项目中使用经证实的古思里试验（1）所获得的结果进行了比较：脱氢酶试验比古思里试验快得多，且底物特异性更高（持续时间分别为2小时和44小时；半乳糖浓度为16.87 mmol/l或更高时的假阳性结果分别为1:42231和1:3519）。然而，它的敏感性较低（半乳糖浓度分别为16.87 mmol/l和1.69 mmol/l），因此两种试验结合似乎适合用于新生儿的大规模筛查。
