通过两种转录因子将RNA聚合酶的两个α亚基羧基末端结构域定位在启动子上。
Positioning of two alpha subunit carboxy-terminal domains of RNA polymerase at promoters by two transcription factors.
作者信息
Murakami K, Owens J T, Belyaeva T A, Meares C F, Busby S J, Ishihama A
机构信息
Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411, Japan.
出版信息
Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11274-8. doi: 10.1073/pnas.94.21.11274.
Abstract
Interactions between the cAMP receptor protein (CRP) and the carboxy-terminal regulatory domain (CTD) of Escherichia coli RNA polymerase alpha subunit were analyzed at promoters carrying tandem DNA sites for CRP binding using a chemical nuclease covalently attached to alpha. Each CRP dimer was found to direct the positioning of one of the two alpha subunit CTDs. Thus, the function of RNA polymerase may be subject to regulation through protein-protein interactions between the two alpha subunits and two different species of transcription factors.
摘要
利用与α亚基共价连接的化学核酸酶，在携带串联CRP结合DNA位点的启动子处分析了环磷酸腺苷受体蛋白（CRP）与大肠杆菌RNA聚合酶α亚基的羧基末端调节结构域（CTD）之间的相互作用。发现每个CRP二聚体可指导两个α亚基CTD之一的定位。因此，RNA聚合酶的功能可能通过两个α亚基与两种不同转录因子之间的蛋白质-蛋白质相互作用受到调控。
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J Mol Biol. 1997 Apr 11;267(4):953-62. doi: 10.1006/jmbi.1997.0902.
2
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9
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