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通过两种转录因子将RNA聚合酶的两个α亚基羧基末端结构域定位在启动子上。

Positioning of two alpha subunit carboxy-terminal domains of RNA polymerase at promoters by two transcription factors.

作者信息

Murakami K, Owens J T, Belyaeva T A, Meares C F, Busby S J, Ishihama A

机构信息

Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411, Japan.

出版信息

Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11274-8. doi: 10.1073/pnas.94.21.11274.

DOI:10.1073/pnas.94.21.11274
PMID:9326599
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC23438/
Abstract

Interactions between the cAMP receptor protein (CRP) and the carboxy-terminal regulatory domain (CTD) of Escherichia coli RNA polymerase alpha subunit were analyzed at promoters carrying tandem DNA sites for CRP binding using a chemical nuclease covalently attached to alpha. Each CRP dimer was found to direct the positioning of one of the two alpha subunit CTDs. Thus, the function of RNA polymerase may be subject to regulation through protein-protein interactions between the two alpha subunits and two different species of transcription factors.

摘要

利用与α亚基共价连接的化学核酸酶，在携带串联CRP结合DNA位点的启动子处分析了环磷酸腺苷受体蛋白（CRP）与大肠杆菌RNA聚合酶α亚基的羧基末端调节结构域（CTD）之间的相互作用。发现每个CRP二聚体可指导两个α亚基CTD之一的定位。因此，RNA聚合酶的功能可能通过两个α亚基与两种不同转录因子之间的蛋白质-蛋白质相互作用受到调控。

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Positioning of two alpha subunit carboxy-terminal domains of RNA polymerase at promoters by two transcription factors.通过两种转录因子将RNA聚合酶的两个α亚基羧基末端结构域定位在启动子上。
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本文引用的文献

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Flexible linker in the RNA polymerase alpha subunit facilitates the independent motion of the C-terminal activator contact domain.RNA聚合酶α亚基中的柔性接头促进了C端激活剂接触结构域的独立运动。
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The two alpha subunits of Escherichia coli RNA polymerase are asymmetrically arranged and contact different halves of the DNA upstream element.大肠杆菌RNA聚合酶的两个α亚基呈不对称排列，并与DNA上游元件的不同半区接触。
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Transcription factor recognition surface on the RNA polymerase alpha subunit is involved in contact with the DNA enhancer element.RNA聚合酶α亚基上的转录因子识别表面参与与DNA增强子元件的接触。
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Protein-protein interactions during transcription activation: the case of the Escherichia coli cyclic AMP receptor protein.转录激活过程中的蛋白质-蛋白质相互作用：以大肠杆菌环腺苷酸受体蛋白为例。
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Location of the C-terminal domain of the RNA polymerase alpha subunit in different open complexes at the Escherichia coli galactose operon regulatory region.大肠杆菌半乳糖操纵子调控区域不同开放复合物中RNA聚合酶α亚基C末端结构域的定位
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