Vial T, Carleer J, Legrain B, Verdier F, Descotes J
Department of Pharmacology, Medical Toxicology and Environmental Medicine, INSERM U80, Faculty of Medicine Lyon-RTH Laënnec, France.
Toxicology. 1997 Oct 19;122(3):213-8. doi: 10.1016/s0300-483x(97)00089-9.
The popliteal lymph node (PLN) assay was proposed to predict possible autoimmune effects of xenobiotics. A preliminary interlaboratory validation study of the PLN assay was conducted in Wistar rats. Three laboratories tested in blind fashion four compounds, namely chlorpromazine, zimeldine, hydrazine and streptozotocin, which were reported to cause autoimmune-like reactions in humans, and one compound, i.e. barbital, which was not, using strictly the same experimental procedure. All tested substances were injected into the hind footpad of rats on day 1, and PLN weight and cellularity were measured on day 8. Comparison of the controlateral PLN was used to calculate weight and cellularity indices. The results were independently analyzed in a fourth laboratory. All four positive compounds were detected by the three laboratories using both weight and cellularity indices, and the negative compound consistently proved negative. Despite variations in absolute values between laboratories, although not significant, these results provide further evidence of the potential predictive value of the PLN assay.
腘淋巴结（PLN）试验被提议用于预测外源性物质可能产生的自身免疫效应。在Wistar大鼠中进行了一项PLN试验的初步实验室间验证研究。三个实验室以盲法测试了四种化合物，即氯丙嗪、齐美利定、肼和链脲佐菌素，据报道这些化合物会在人类中引发类似自身免疫的反应，还测试了一种不会引发此类反应的化合物，即巴比妥，所有实验室都严格采用相同的实验程序。所有受试物质在第1天注射到大鼠的后足垫，在第8天测量PLN的重量和细胞数量。通过对侧PLN的比较来计算重量和细胞数量指数。结果在第四个实验室进行独立分析。三个实验室使用重量和细胞数量指数均检测到了所有四种阳性化合物，而阴性化合物始终被证明为阴性。尽管各实验室之间的绝对值存在差异，虽然差异不显著，但这些结果进一步证明了PLN试验具有潜在的预测价值。
