Russell K E, Perkins P C, Grindem C B, Walker K M, Sellon D C
Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
Am J Vet Res. 1997 Oct;58(10):1092-6.
To evaluate a method for detecting thiazole orange-positive (TO+, reticulated) platelets in equine blood, using flow cytometry.
16 healthy, equine infectious anemia virus (EIAV)-negative horses and ponies; 9 thrombocytopenic, EIAV-positive horses and ponies; and 2 thrombocytopenic, EIAV-negative horses.
Blood from healthy and thrombocytopenic horses was collected by jugular venipuncture. Appropriate sample requirement and incubation time for the assay were evaluated, using blood anticoagulated with EDTA or sodium citrate, or platelet-rich plasma in sodium citrate. The sample of blood or platelet-rich plasma was incubated with thiazole orange, and flow cytometric analysis was performed. Percentage of circulating TO+ platelets was determined from fluorescence (FL-1) logarithmic histograms.
Healthy ponies (n = 9) had 1.28 to 2.83% (mean +/- SD, 2.03 +/- 0.50%) and horses (n = 7) had 0.9 to 3.44% (2.12 +/- 1.14%) TO+ platelets in circulation. Thrombocytopenic ponies (n = 7) had 11.14 to 48.41% (26.51 +/- 11.99%) and thrombocytopenic horses (n = 4) had 2.33 to 8.52% (6.19 +/- 2.68%) TO+ platelets in circulation. Mean platelet counts for the thrombocytopenic ponies and horses were 24,400 +/- 20,500 and 39,300 +/- 13,500 platelets/microliters, respectively (reference range, 94,000 to 232,000 platelets/ microliters).
Thiazole orange-positive platelets can be detected in equine blood and percentages of TO+ platelets are increased in thrombocytopenic horses.
Enumeration of TO+ platelets may prove to be a helpful noninvasive clinical measurement of bone marrow platelet production and aid in the assessment of platelet kinetics in thrombocytopenic horses.
采用流式细胞术评估一种检测马血液中噻唑橙阳性（TO+，网状）血小板的方法。
16匹健康、马传染性贫血病毒（EIAV）阴性的马和矮种马；9匹血小板减少、EIAV阳性的马和矮种马；以及2匹血小板减少、EIAV阴性的马。
通过颈静脉穿刺采集健康和血小板减少马匹的血液。使用用乙二胺四乙酸（EDTA）或柠檬酸钠抗凝的血液，或柠檬酸钠中的富血小板血浆，评估该检测的适当样本要求和孵育时间。将血液或富血小板血浆样本与噻唑橙孵育，然后进行流式细胞术分析。根据荧光（FL-1）对数直方图确定循环中TO+血小板的百分比。
健康矮种马（n = 9）循环中TO+血小板的比例为1.28%至2.83%（平均值±标准差，2.03±0.50%），健康马（n = 7）为0.9%至3.44%（2.12±1.14%）。血小板减少的矮种马（n = 7）循环中TO+血小板的比例为11.14%至48.41%（26.51±11.99%），血小板减少的马（n = 4）为2.33%至8.52%（6.19±2.68%）。血小板减少的矮种马和马的平均血小板计数分别为每微升24,400±20,500和39,300±13,500个血小板（参考范围为每微升94,000至232,000个血小板）。
马血液中可检测到噻唑橙阳性血小板，血小板减少的马中TO+血小板的百分比增加。
TO+血小板的计数可能被证明是一种有助于无创临床测量骨髓血小板生成的方法，并有助于评估血小板减少马匹的血小板动力学。
