Neelakantan L, Kostenbauder H B
J Pharm Sci. 1976 May;65(5):740-2. doi: 10.1002/jps.2600650530.
A simplified procedure is described for an electron-capture GLC determination of phenylpropanolamine in blood plasma. The method is based on derivatization of phenylpropanolamine with pentafluorobenzaldehyde at room temperature without prior extraction of the drug from plasma. The derivative, pentafluorophenyloxazolidine, is readily extracted from plasma into a small volume of hexane. Samples usually can be injected directly into the gas chromatograph without concentration of the hexane solution. Data are presented to illustrate the suitability of the method for dosage form bioavailability evaluation from plasma phenylpropanolamine levels achieved after acute oral administration of a typical dosage form.
本文描述了一种用于血浆中苯丙醇胺的电子捕获气相色谱测定的简化程序。该方法基于在室温下用五氟苯甲醛对苯丙醇胺进行衍生化，无需事先从血浆中提取药物。衍生物五氟苯基恶唑烷很容易从血浆中萃取到少量己烷中。样品通常无需浓缩己烷溶液即可直接注入气相色谱仪。文中给出的数据表明该方法适用于根据典型剂型急性口服给药后血浆中苯丙醇胺水平评估剂型生物利用度。
