Mielke S, Meden H, Raab T, Wuttke W, Kuhn W
Department of Obstetrics and Gynecology, University of Göttingen, Germany.
Anticancer Res. 1997 Jul-Aug;17(4B):3125-7.
The 105 kDa extracellular domain of c-erbB-2 encoded protein p185 is detectable in serum. Elevated p105 serum levels were found in patients with breast and ovarian cancer and in pregnancy. The effects of interfering and influencing factors on the measurement are still unclear.
We used an ELISA based on the monoclonal capture antibody OD-3 and investigated possible interfering factors, like changing transporting and storing conditions of blood and serum samples. In order to evaluate a normal range of p105 serum values we examined 71 healthy women.
In our tests we found no influence on the reproducibility of results by changing transporting and storing conditions. We could not find any dependency of p105 serum values on the menstrual cycle or the age of controls. Postmenopausal women showed significantly higher serum values than premenopausal women (p = 0.0127).
Clinical interpretation of female p105 serum values requires comparison with normal ranges when considering the menopause as an influencing factor.
c-erbB-2编码蛋白p185的105 kDa细胞外结构域可在血清中检测到。在乳腺癌和卵巢癌患者以及孕期女性中发现血清p105水平升高。干扰因素和影响因素对该测量的作用仍不清楚。
我们使用基于单克隆捕获抗体OD-3的酶联免疫吸附测定法（ELISA），并研究了可能的干扰因素，如血液和血清样本运输及储存条件的改变。为评估p105血清值的正常范围，我们检测了71名健康女性。
在我们的测试中，发现改变运输和储存条件对结果的可重复性没有影响。我们未发现p105血清值与月经周期或对照者年龄之间存在任何相关性。绝经后女性的血清值显著高于绝经前女性（p = 0.0127）。
在将绝经视为影响因素时，对女性p105血清值进行临床解读需要与正常范围进行比较。
