人尿中异烟酰异丙肼的鉴定及定量气相色谱测定

Identification and quantitative GLC determination of iproniazid in human urine.

作者信息

de Sagher R M, De Leenheer A P, Claeys A E

出版信息

J Pharm Sci. 1976 Jun;65(6):878-82. doi: 10.1002/jps.2600650619.

Abstract

A GLC method for the specific identification and quantitation of iproiazid, a potent monamine oxidase inhibitor drug, is described. The free drug isolated from urine samples by two-phase extraction reinforced with salting out. Identification and quantitative determination at the microgram level are done on aliquots of the chloroform extract by GLC, using the 2-butyl analog as an internal standard. Iproniazid and potentially interfering compounds present in the extracts are also identified by GLC-mass spectrometry and TLC for supporting evidence of the GLC method's specificity.

摘要

本文描述了一种用于特异性鉴定和定量测定异烟酰异丙肼（一种有效的单胺氧化酶抑制剂药物）的气相色谱法。通过盐析强化的两相萃取从尿液样本中分离出游离药物。使用2-丁基类似物作为内标，通过气相色谱法对氯仿提取物的等分试样进行微克级别的鉴定和定量测定。提取物中存在的异烟酰异丙肼和潜在干扰化合物也通过气相色谱-质谱联用仪和薄层色谱法进行鉴定，以支持气相色谱法特异性的证据。