García R, Ballesteros L M, Hernández-Pérez O, Rosales A M, Espinosa R, Soto H, Díaz de León L, Rosado A
Laboratorio de Bioquímica Molecular, Unidad de Investigación Médica en Bioquímica, Instituto Mexicano del Seguro Social, México DF, Mexico.
Anim Reprod Sci. 1997 Jun;47(3):211-28. doi: 10.1016/s0378-4320(96)01637-5.
Metalloproteinases are an important group of hydrolytic enzymes which participate in interstitial matrix degradation during tissue remodelling processes and therefore may be required during follicular growth and maturation. The activity of metalloproteinases (collagenases, gelatinase, and Pz-peptidase), was measured during growth, maturation and atresia of goat antral follicles. These follicles (n = 67) were separated by size and also classified into four groups: non-atretic (Group I); early atretic (Stage I) (Group II); moderately atretic (Stage II) (Group IIIa); and, late atretic (Stage III) (Group IIIb). Pz-peptidase was greater in granulosa than in thecal cells, and almost absent in follicular fluid. In non-atretic follicles, activity in granulosa cells increased with increasing follicle size, whereas activity peaked in 3-6 mm follicles in thecal cells. Atresia was associated with declining activity in thecal cells from follicles in the 3-6 mm range and in granulosa cells from the > 6 mm range. Interstitial collagenase activity was significant and similar in granulosa and thecal cell extracts and low in follicular fluid from non-atretic follicles. Activity increased significantly in thecal cells, but decreased significantly in granulosa cells from large (> 6 mm) non-atretic follicles. Atresia was associated with declining activity in both types cells and increasing activity in follicular fluid. Gelatinase activity was some times associated with five regions corresponding to molecular weights of 22.1, 30.7, 39.6, 63.8 and 71.4 kDa, and rarely at 91.3 and 81.2 kDa. Overall activity declined with atresia in thecal cells from follicles in the 3-6 mm range, but not in those > 6 mm. In granulosa cells from follicles 3-6 mm, activity varied widely with stage of atresia, while in cells from follicles > 6 mm, activity was greatly increased in atretic follicles.
金属蛋白酶是一组重要的水解酶，在组织重塑过程中参与细胞间质基质的降解，因此在卵泡生长和成熟过程中可能是必需的。在山羊有腔卵泡的生长、成熟和闭锁过程中，测定了金属蛋白酶（胶原酶、明胶酶和Pz肽酶）的活性。这些卵泡（n = 67）按大小进行分离，并分为四组：非闭锁卵泡（I组）；早期闭锁卵泡（I期）（II组）；中度闭锁卵泡（II期）（IIIa组）；晚期闭锁卵泡（III期）（IIIb组）。Pz肽酶在颗粒细胞中的含量高于卵泡膜细胞，在卵泡液中几乎不存在。在非闭锁卵泡中，颗粒细胞中的活性随卵泡大小的增加而增加，而卵泡膜细胞中的活性在3 - 6 mm卵泡中达到峰值。闭锁与3 - 6 mm范围内卵泡的卵泡膜细胞以及大于6 mm范围内卵泡的颗粒细胞活性下降有关。间质胶原酶活性在颗粒细胞和卵泡膜细胞提取物中显著且相似，在非闭锁卵泡的卵泡液中较低。在大的（> 6 mm）非闭锁卵泡中，卵泡膜细胞中的活性显著增加，但颗粒细胞中的活性显著降低。闭锁与两种类型细胞的活性下降以及卵泡液中活性增加有关。明胶酶活性有时与对应分子量为22.1、30.7、39.6、63.8和71.4 kDa的五个区域相关，很少与91.3和81.2 kDa相关。在3 - 6 mm范围内卵泡的卵泡膜细胞中，总体活性随闭锁而下降，但在大于6 mm的卵泡中则不然。在3 - 6 mm卵泡的颗粒细胞中，活性随闭锁阶段变化很大，而在大于6 mm卵泡的细胞中，闭锁卵泡中的活性大大增加。
