College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
J Zhejiang Univ Sci B. 2010 May;11(5):307-14. doi: 10.1631/jzus.B0900391.
There is a lack of appropriate classification criteria for the determination of atretic follicles in guinea pigs. In the present study, new criteria were established based on the latest morphologic criteria for cell death proposed by the Nomenclature Committee on Cell Death (NCCD) in 2009. Ovaries of guinea pigs were sampled on different stages of estrous cycle, and the morphologic observations of atretic follicles were investigated in serial sections. The results showed that the process of follicular atresia could be classified into four continuous stages: (1) the granulosa layer became loose, and some apoptotic bodies began to appear; (2) the granulosa cells were massively eliminated; (3) the theca interna cells differentiated; and (4) the residual follicular cells degenerated. In addition, the examination revealed that these morphologic criteria were accurate and feasible. In conclusion, this study provides new criteria for the classification of atretic follicles in guinea pigs, and this knowledge can inform future research in the area.
在豚鼠中，存在确定闭锁卵泡缺乏适当的分类标准的问题。在本研究中，根据细胞死亡命名委员会（NCCD）在 2009 年提出的最新形态学细胞死亡标准，建立了新的标准。在不同动情周期阶段采集豚鼠的卵巢，并在连续切片中研究闭锁卵泡的形态学观察。结果表明，卵泡闭锁过程可分为四个连续阶段：（1）颗粒层变得松散，一些凋亡小体开始出现；（2）颗粒细胞大量被消除；（3）内膜细胞分化；和（4）剩余的卵泡细胞退化。此外，检查结果表明这些形态学标准是准确和可行的。总之，本研究为豚鼠闭锁卵泡的分类提供了新标准，这一知识可以为该领域的未来研究提供信息。
