Brintnell B, Hey Y, Jones D, Hoggard N, James L, Varley J M
CRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Manchester, UK.
Somat Cell Mol Genet. 1997 Mar;23(2):153-7. doi: 10.1007/BF02679974.
Chromosome region 1p13 is known to show loss of heterozygosity (LOH) in a number of human tumor types, including breast. We have generated a contig comprising YACs and BACs spanning part of 1p13.1 which includes the smallest region of overlapping loss identified in our earlier studies. The contig is anchored to the genetic map by a number of microsatellite markers, and by the use of CEPH YACs. We have excluded a number of candidate genes from this region, and we have oriented the contig with respect to the centromere and a number of other genes and markers on 1p13. This resource will be valuable in mapping the target for LOH in breast and other tumors, and may also be useful for the genetic analysis of other genes or diseases known to map to this region.
已知染色体区域1p13在包括乳腺癌在内的多种人类肿瘤类型中显示杂合性缺失（LOH）。我们构建了一个由酵母人工染色体（YAC）和细菌人工染色体（BAC）组成的重叠群，其跨越1p13.1的一部分，其中包括我们早期研究中确定的最小重叠缺失区域。该重叠群通过多个微卫星标记以及使用CEPH YAC锚定到遗传图谱上。我们已经排除了该区域的一些候选基因，并已将该重叠群相对于着丝粒以及1p13上的其他一些基因和标记进行了定向。这一资源对于确定乳腺癌和其他肿瘤中LOH的靶点将是有价值的，也可能有助于对已知定位于该区域的其他基因或疾病进行遗传分析。
