Keithly J S
J Protozool. 1976 May;23(2):244-5. doi: 10.1111/j.1550-7408.1976.tb03763.x.
Intracardial injection of hamsters with from 5 to 114 million amastigotes or promastigotes of Leishmania donovani and screening of the 8th-day liver impression smears, provides a rapid and reproducible method for assaying infectivity. Amastigotes are at least 10X more infective than promastigotes, and log-phase promastigotes act as a single infective population for hamsters.
给仓鼠进行心内注射500万至1.14亿个杜氏利什曼原虫的无鞭毛体或前鞭毛体,并对第8天的肝脏印片进行筛查,提供了一种快速且可重复的检测感染性的方法。无鞭毛体的感染性至少比前鞭毛体高10倍,对数期前鞭毛体对仓鼠而言是单一感染群体。