Fahey J R, Herman R
Infect Immun. 1985 Aug;49(2):447-51. doi: 10.1128/iai.49.2.447-451.1985.
The relationship between the development of acquired cell-mediated immunity and the concomitant level of specific delayed hypersensitivity (DH) in C57BL/6J mice infected with the intracellular protozoan parasite Leishmania donovani was studied. It was determined that the intradermal injection of Leishmania antigen (heat- or phenol-killed flagellated forms of L. donovani) could elicit a DH response as early as day 10 and as late as day 120 postinfection in mice infected by the intravenous route. Dose-response studies showed that there is an inverse relationship between the size of the infecting dose of parasites and the magnitude of the DH response. Thus, increasing the degree of infection depressed the DH response. This depression, however, could be abrogated by treatment of mice with cyclophosphamide (201 mg/kg) before intradermal injection of antigen.
研究了感染细胞内原生动物寄生虫杜氏利什曼原虫的C57BL/6J小鼠获得性细胞介导免疫的发展与伴随的特异性迟发型超敏反应（DH）水平之间的关系。结果表明，皮内注射利什曼原虫抗原（热灭活或酚灭活的杜氏利什曼原虫鞭毛体形式）在静脉感染小鼠中最早可在感染后第10天、最晚在第120天引发DH反应。剂量反应研究表明，寄生虫感染剂量的大小与DH反应的强度呈负相关。因此，感染程度的增加会抑制DH反应。然而，在皮内注射抗原前用环磷酰胺（201 mg/kg）治疗小鼠可消除这种抑制作用。
