Kamino K, Ogawa M, Uyesaka N, Inouye A
J Membr Biol. 1976 May;26(4):345-56. doi: 10.1007/BF01868882.
Ruthenium red combines with isolated synaptosomes, resulting in strong inhibition of their Ca2+-binding. In isotonic saline media, however, the dye-induced inhibition of Ca2+-binding is significantly greater than that expected for the amount of bound dye and Hill's exponent of the Ca2+-binding decreases to 1 with an increase in the amount of the dye bound. On the other hand in isotonic mannitol-sucrose solution, inhibition of synaptosmal Ca2+-binding brought about by the dye is proportional to the amount of dye bound. Based on these results, the effects of the dye on the co-operative nature of synaptosomal Ca2+-binding is discussed.
钌红与分离出的突触体结合，导致其钙结合能力受到强烈抑制。然而，在等渗盐溶液中，染料诱导的钙结合抑制作用明显大于根据结合染料量预期的抑制作用，并且随着结合染料量的增加，钙结合的希尔系数降至1。另一方面，在等渗甘露醇 - 蔗糖溶液中，该染料引起的突触体钙结合抑制作用与结合染料量成正比。基于这些结果，讨论了该染料对突触体钙结合协同性质的影响。
