Bozhkov A I, Malyshev A B, Shentseva E A
Institute of Biology, Kharkov State University, Ukraine.
Biochemistry (Mosc). 1997 Jul;62(7):793-800.
A 12 kD protein (IHP-12) was isolated from the cytosol of liver cells; IHP-12 inhibited proliferation of the liver cells by 50% when injected at 2.5 mg/100 g animal weight. Repeated injections of IHP-12 induced resistance of the liver cells to its anti-proliferative effect. After intraperitoneal injection, IHP-12 is localized in the cytosol and to a lesser extent, in cell nuclei. IHP-12 is degraded in the cytosol but in nuclei it retained its native structure for at least 8 h. The mechanism of induced resistance to IHP-12 was not related to a change in cell binding of IHP-12, its distribution in cellular compartments, and intracellular metabolism. The induced resistance may be associated with a change in the direction of IHP-12 activity, i.e., change in its function in the animal.
从肝细胞胞质溶胶中分离出一种12 kD的蛋白质（IHP - 12）；当以2.5 mg/100 g动物体重注射时，IHP - 12可使肝细胞增殖抑制50%。重复注射IHP - 12可诱导肝细胞对其抗增殖作用产生抗性。腹腔注射后，IHP - 12定位于胞质溶胶中，在细胞核中的定位程度较低。IHP - 12在胞质溶胶中降解，但在细胞核中至少8小时保持其天然结构。对IHP - 12诱导抗性的机制与IHP - 12的细胞结合变化、其在细胞区室中的分布以及细胞内代谢无关。诱导抗性可能与IHP - 12活性方向的改变有关，即其在动物体内功能的改变。
