Pietu G, Chany E, Molimard R, Frayssinet C
Biomedicine. 1978 May-Jun;28(3):169-75.
The control of organ cell multiplication by a negative feed-back mechanism implies that factors of the inhibitory mechanism are present in the blood and are produced by the target organ itself. We have examined the inhibitors which are present in the cytosol of adult rat liver as well as in efferent fluid from an isolated perfused organ. These two materials yielded predominantly protein molecules having in common a molecular weight of approximately 80 000 daltons and an isoelectric point between pH 8 and 9. They inhibit both the multiplication of LF hepatoma cells in vitro and the DNA synthesis in the remnant liver following partial hepatectomy in rats. The inhibitors were compared immunologically. When antibodies against the cytosol inhibitor were tested with the Ouchterlong technique against either the original antigen or the inhibitory fraction isolated from the perfusion effluent, a common precipitation line appeared. The inhibitory activity of both the cytosol and the perfusate was neutralized by prior incubation with anti-cytosol antibodies. These results, which strongly suggest a common inhibitor in the cytosol and the perfusate, strongly support the hypothesis of a feed-back mechanism for the control of liver cell proliferation.
通过负反馈机制对器官细胞增殖进行控制意味着，抑制机制的因子存在于血液中，且由靶器官自身产生。我们已经检测了成年大鼠肝脏胞质溶胶以及离体灌注器官的流出液中的抑制剂。这两种物质主要产生分子量约为80000道尔顿、等电点在pH 8至9之间的蛋白质分子。它们既能抑制体外LF肝癌细胞的增殖，也能抑制大鼠部分肝切除术后残余肝脏中的DNA合成。对这些抑制剂进行了免疫学比较。当用Ouchterlong技术针对原抗原或从灌注流出物中分离出的抑制部分检测抗胞质溶胶抑制剂的抗体时，出现了一条共同的沉淀线。通过预先与抗胞质溶胶抗体孵育，胞质溶胶和灌注液的抑制活性均被中和。这些结果强烈表明胞质溶胶和灌注液中存在共同的抑制剂，有力地支持了肝细胞增殖控制的反馈机制假说。
