Tagashira S, Harada H, Katsumata T, Itoh N, Nakatsuka M
Sumitomo Pharmaceuticals Research Center, Osaka, Japan.
Gene. 1997 Sep 15;197(1-2):399-404. doi: 10.1016/s0378-1119(97)00187-x.
We cloned the mouse homolog of FGF10, which was recently reported as a new member of the FGF family. The predicted molecular mass of this molecule is 23.6 kDa, and both nucleotide and amino acid sequences show high degrees of similarity with those of the rat. Examination of mouse FGF10 mRNA expression in various tissues and developmental stages by Northern hybridization revealed tissue- and developmental stage-specific expression of the gene. Similarly to the rat counterpart, mouse FGF10 mRNA (4.5 kb) was expressed relatively abundantly in embryos and the lung, and at much lower levels in brain and heart. In addition, a shorter transcript (1.3 kb) is expressed only in testis. Considering the high similarity in primary structure between FGF10 and FGF7 (known as keratinocyte growth factor; KGF), we also examined the gene expression of FGF10 during wound healing using a mouse model. FGF10 mRNA was highly induced 1 day after injury and decreased rapidly by 3 days. This suggests that FGF10 is a primary factor in the process of wound healing similarly to other growth factors such as TGF alpha and FGF7.
我们克隆了FGF10的小鼠同源物，它最近被报道为FGF家族的一个新成员。该分子的预测分子量为23.6 kDa，其核苷酸和氨基酸序列与大鼠的序列具有高度相似性。通过Northern杂交检测小鼠FGF10 mRNA在各种组织和发育阶段的表达，揭示了该基因在组织和发育阶段的特异性表达。与大鼠对应物相似，小鼠FGF10 mRNA（4.5 kb）在胚胎和肺中相对大量表达，而在脑和心脏中的表达水平则低得多。此外，一个较短的转录本（1.3 kb）仅在睾丸中表达。考虑到FGF10与FGF7（已知为角质形成细胞生长因子；KGF）在一级结构上的高度相似性，我们还使用小鼠模型研究了伤口愈合过程中FGF10的基因表达。FGF10 mRNA在损伤后1天被高度诱导，并在3天时迅速下降。这表明FGF10与其他生长因子如TGFα和FGF7一样，是伤口愈合过程中的一个主要因子。
