Romero L M, Wingfield J C
Department of Zoology, University of Washington, Seattle 98195, USA.
Physiol Behav. 1997 Nov;62(5):1109-12. doi: 10.1016/s0031-9384(97)00264-3.
Recently, there has been an increase in the interest in various aspects of the biology of small passerine birds. In order to apply many modern neurobiological techniques, it would be useful to be able to surgically inject hormones and drugs directly into passerine brains. We describe a novel and cost-effective stereotaxic-like technique for injecting substances into the lateral ventricles of small passerine birds. The technique is rapid, reducing the period of anesthesia, and reliable. In addition, using this technique, we injected [3H]cysteine (to label newly synthesized proteins) with small doses of colchicine. Colchicine decreased the content of newly synthesized protein in the median eminence, thus suggesting that colchicine inhibited protein transport. The technique should prove useful in a number of neurobiologial applications.
最近，人们对小型雀形目鸟类生物学各个方面的兴趣有所增加。为了应用许多现代神经生物学技术，能够通过手术将激素和药物直接注射到雀形目鸟类的大脑中将会很有用。我们描述了一种新颖且经济高效的类似立体定位的技术，用于将物质注射到小型雀形目鸟类的侧脑室中。该技术操作迅速，减少了麻醉时间，且可靠。此外，使用该技术，我们将[3H]半胱氨酸（用于标记新合成的蛋白质）与小剂量秋水仙碱一起注射。秋水仙碱降低了正中隆起中新合成蛋白质的含量，因此表明秋水仙碱抑制了蛋白质转运。该技术在许多神经生物学应用中应会证明是有用的。
