Kohlhaas M, Carstens J, Richard G
Universitäts-Augenklinik Hamburg-Eppendorf.
Ophthalmologe. 1997 Jul;94(7):475-80. doi: 10.1007/s003470050142.
The effects of deep excimer laser ablations on the corneal endothelial cell count are still unknown.
In an in-vitro study the endothelial cell counts of 125 pig corneas were examined after excimer laser ablation. Five groups of 25 eyes reach, one control group and four groups with 3, 10, 20, and 35 D of ablation were examined. After the laser treatment, a corneoscleral disc was prepared and stored for up 5 days in an organ culture medium. The endothelial cell density of 5 corneas in each group was measured in a period of 5 days with a phase contrast microscope.
Significant cell loss, 13-25%, was measured in each group after 5 days. There were no significant differences between the ablated corneas and the control group.
Deep excimer laser ablations do not significantly reduce the endothelial cell count.
准分子激光深层消融对角膜内皮细胞计数的影响尚不清楚。
在一项体外研究中，对125只猪角膜进行准分子激光消融后检查其内皮细胞计数。研究了五组，每组25只眼，一组为对照组，另外四组分别进行了3D、10D、20D和35D的消融。激光治疗后，制备角膜巩膜瓣并在器官培养基中保存长达5天。在5天内用相差显微镜测量每组5只角膜的内皮细胞密度。
5天后，每组均出现了13% - 25%的显著细胞损失。消融角膜与对照组之间无显著差异。
准分子激光深层消融不会显著降低内皮细胞计数。
