Lambert R W, Anderson J A, Heitzmann J, Sutherland C J, Moore M M, Binder P S
National Vision Research Institute, San Diego, Calif, USA.
Arch Ophthalmol. 1996 Dec;114(12):1499-505. doi: 10.1001/archopht.1996.01100140697010.
To determine the possibility of endothelial cell damage after excimer laser ablation.
Endothelial cell densities and morphology of human corneas after photoablations or mechanical keratectomy were compared with those of the untreated mates after 1 week of culture with or without serum.
Corneas cultured in serum-free medium after ablation to a depth of 150 microns showed endothelial cell densities reduced to 60% of untreated, mate corneas; ultrastructural analysis showed endothelial cell damage not seen in untreated mates. Corneas ablated to the same depth and cultured in serum-enriched medium showed no endothelial cell density loss, nor did corneas cultured in serum-free medium after an ablation to a depth of 50 microns or mechanical keratectomies averaging 95 microns.
Endothelial cell loss in deep laser resections may be prevented by factor(s) in fetal bovine serum. The apparent lack of cell loss in clinical studies may be related to the protective action of similar factors in aqueous humor.
确定准分子激光消融术后内皮细胞损伤的可能性。
将光消融或机械角膜切除术后人角膜的内皮细胞密度和形态与未经处理的配对角膜在有或无血清培养1周后的情况进行比较。
消融至150微米深度后在无血清培养基中培养的角膜，其内皮细胞密度降至未经处理的配对角膜的60%；超微结构分析显示未经处理的配对角膜未见内皮细胞损伤。消融至相同深度并在富含血清的培养基中培养的角膜，内皮细胞密度没有损失，消融至50微米深度后在无血清培养基中培养的角膜或平均95微米的机械角膜切除术的角膜也没有内皮细胞密度损失。
胎牛血清中的某些因素可能预防深层激光切除术中的内皮细胞丢失。临床研究中明显缺乏细胞丢失可能与房水中类似因素的保护作用有关。
