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[通过聚合酶链反应对生牛奶中产志贺样毒素大肠杆菌的调查]

[Investigations of raw milk for Shiga-like toxin producing Escherichia coli by the polymerase chain reaction].

作者信息

Mäde D, Stark R

机构信息

Landesveterinär- und Lebensmitteluntersuchungsamt Halle.

出版信息

Dtsch Tierarztl Wochenschr. 1996 Dec;103(12):511-2.

PMID:9333541
Abstract

This is a report about the occurrence of Shiga-like toxin (SLT) producing Escherichia coli in milk in southern Sachsen-Anhalt. Samples were taken from tanks of milk at the farms. The samples were investigated by polymerase chain reaction as recommended by the "Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin". Using PCR, in 11% of the tank samples sequences coding for SLT I and/or SLT II were detected. 75% of the as positive detected samples had eae gen. Some cows in these farms spread potentially enterohemorrhagic E. coli. These farms should not sell milk for raw consumption.

摘要

这是一份关于萨克森-安哈尔特州南部牛奶中产生志贺样毒素(SLT)的大肠杆菌的发生情况报告。样本取自农场的牛奶储存罐。按照“联邦消费者健康保护和兽医学研究所”的建议,通过聚合酶链反应对样本进行了检测。使用聚合酶链反应,在11%的储存罐样本中检测到了编码SLT I和/或SLT II的序列。75%检测呈阳性的样本含有eae基因。这些农场的一些奶牛传播了潜在的肠出血性大肠杆菌。这些农场不应出售供生饮的牛奶。

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