Takeuchi A, Binn L N, Jervis H R, Keenan K P, Hildebrandt P K, Valas R B, Bland F F
Lab Invest. 1976 Jun;34(6):539-49.
Neonatal dogs, inoculated orally with coronavirus 1-71, grown in canine kidney cell cultures, developed diarrhea and a severe enteritis characterized by atrophy of the villi, changes in the enterocytes, and accelerated epithelial cell loss. Electron microscopy of the mucosal epithelium, 4 days after challenge, showed that the virus penetrated into the enterocytes between microvilli, possibly by pinocytotic mechanism. In the enterocytes, virions were most often enclosed, singly or in groups, in cytoplasmic vesicles. They were less frequently found in the cisternae of the Golgi apparatus, the endoplasmic reticulum, or in the dilated perinuclear space and only rarely, free in the cytoplasm. Virions replicated by budding only on the smooth.membranes of the cytoplasmic vesicles. The infected cells showed a variety of cytopathic effects, some nonspecific, such as disruption of the microvilli, loss of density of the cytoplasm, presence of lipid inclusions, alteration of mitochondria, and dilation of the endoplasmic reticulum and Golgi cisternae and of the perinuclear space. Other cytopathic effects, characteristic of the coronavirus infection, consisted of formation of dense filamentous structures and of membrane-bound bodies. Progeny virions appeared to discharge into the gut lumen through the disrupted cell membranes of infected enterocytes still in situ or following their premature shedding.
新生犬经口接种在犬肾细胞培养物中培养的冠状病毒1-71后，出现腹泻和严重肠炎，其特征为绒毛萎缩、肠上皮细胞变化以及上皮细胞脱落加速。攻毒4天后对黏膜上皮进行电子显微镜检查发现，病毒可能通过胞饮机制进入微绒毛之间的肠上皮细胞。在肠上皮细胞中，病毒粒子最常单个或成组地被包裹在细胞质小泡中。在高尔基体、内质网的池或扩张的核周间隙中较少发现它们，在细胞质中则极为罕见。病毒粒子仅通过在细胞质小泡的光滑膜上出芽进行复制。受感染的细胞表现出多种细胞病变效应，一些是非特异性的，如微绒毛破坏、细胞质密度降低、脂滴存在、线粒体改变以及内质网、高尔基体池和核周间隙扩张。其他冠状病毒感染特有的细胞病变效应包括致密丝状结构和膜结合体的形成。子代病毒粒子似乎通过仍在位的受感染肠上皮细胞的破裂细胞膜或在其过早脱落之后排入肠腔。
