Kazanji M, Ibrahim F, Fiette L, Bomford R, De Thé G
Unité d'Epidémiologie des Virus Oncogènes, Institut Pasteur, Paris, France.
Int J Cancer. 1997 Sep 26;73(1):131-6. doi: 10.1002/(sici)1097-0215(19970926)73:1<131::aid-ijc20>3.0.co;2-i.
Three aspects of the rat model of HTLV-I/II infection were investigated. (i) The efficacy of HTLV-I-transformed rat cell lines in infecting different strains of rats: WKY and Lewis HTLV-I-transformed cell lines were injected into adult WKY, Lewis and Brown Norway rats, representing syngeneic and allogeneic combinations. The HTLV-I provirus was not detected in peripheral-blood mononuclear cells (PBMC) from these rats 18 weeks after inoculation, showing that HTLV-I-transformed rat cells are not suitable for virus challenge in vaccination experiments. Rats inoculated with Lewis HTLV-I-transformed cells produced an antibody response to HTLV-I, which was higher in allogeneic (WKY and Brown Norway) than in syngeneic rats. (ii) The susceptibility of rats to HTLV-II infection: After human HTLV-II-producing cells (MO) were injected into adult WKY rats, the HTLV-II provirus was detected in PBMC 12 weeks later. Sequencing of a portion of this provirus confirmed its identity with the HTLV-II from MO cells. (iii) The role of MHC haplotype in susceptibility to neurological disease in rats inoculated as newborns with HTLV-I: The hypothesis that the RT-Ik haplotype confers susceptibility was tested by inoculating newborn OKA (RT-Ik), WKY (RT-Il), Lewis (RT-Il) and Fischer 344 (RT-I lvl) rats with human HTLV-I-producing cells (MT-2). Eighteen months later, only the WKY rats showed histological abnormality of the spinal cord, without clinical paralysis. Fischer 344 rats developed cutaneous tumors and OKA rats mammary tumors. The HTLV-I provirus was not detected in these tumors.
对人嗜T淋巴细胞病毒I/II（HTLV-I/II）感染大鼠模型的三个方面进行了研究。（i）HTLV-I转化的大鼠细胞系感染不同品系大鼠的效果：将WKY和Lewis HTLV-I转化细胞系注射到成年WKY、Lewis和挪威棕色大鼠体内，分别代表同基因和异基因组合。接种18周后，在这些大鼠的外周血单个核细胞（PBMC）中未检测到HTLV-I前病毒，表明HTLV-I转化的大鼠细胞不适用于疫苗接种实验中的病毒攻击。接种Lewis HTLV-I转化细胞的大鼠产生了针对HTLV-I的抗体反应，异基因（WKY和挪威棕色大鼠）中的反应高于同基因大鼠。（ii）大鼠对HTLV-II感染的易感性：将产生人HTLV-II的细胞（MO）注射到成年WKY大鼠体内后，12周后在PBMC中检测到HTLV-II前病毒。对该前病毒的一部分进行测序，证实其与MO细胞中的HTLV-II一致。（iii）MHC单倍型在新生期接种HTLV-I的大鼠对神经疾病易感性中的作用：通过给新生的OKA（RT-Ik）、WKY（RT-Il）、Lewis（RT-Il）和Fischer 344（RT-I lvl）大鼠接种产生人HTLV-I的细胞（MT-2），来检验RT-Ik单倍型赋予易感性的假设。18个月后，只有WKY大鼠出现脊髓组织学异常，但无临床瘫痪。Fischer 344大鼠发生皮肤肿瘤，OKA大鼠发生乳腺肿瘤。在这些肿瘤中未检测到HTLV-I前病毒。
