Delebecque Frédéric, Pramberger Karin, Prévost Marie-Christine, Brahic Michel, Tangy Frédéric
Unité des Virus Lents, URA 1930 CNRS, Institut Pasteur, Paris, France.
J Virol. 2002 Aug;76(15):7883-9. doi: 10.1128/jvi.76.15.7883-7889.2002.
We constructed a chimeric human T-cell lymphotropic virus type 1 (HTLV-1) provirus in which the original envelope precursor sequence was replaced by that of ecotropic Moloney murine leukemia virus (Mo-MuLV). Chimeric particles produced by transient transfection of this chimeric provirus were infectious for murine cells, such as NIH 3T3 fibroblasts, lymphoid EL4 cells, and primary CD4(+) T lymphocytes, whereas HTLV-1 particles were not. The infectivity of chimeric particles increased 10 times when the R peptide located at the carboxy terminus of the MuLV envelope glycoprotein was deleted. Primary murine CD4(+) T lymphocytes, infected by the Delta R chimeric virus, released particles that could spread the infection to other naive murine lymphoid cells. This chimeric virus, with the Mo-MuLV envelope glycoprotein and the replication characteristics of HTLV-1, should be useful in studying the pathogenesis of HTLV-1 in a mouse model.
我们构建了一种嵌合型1型人类嗜T细胞病毒（HTLV-1）前病毒，其中原始的包膜前体序列被亲嗜性莫洛尼鼠白血病病毒（Mo-MuLV）的序列所取代。通过瞬时转染这种嵌合型前病毒产生的嵌合颗粒对鼠细胞具有感染性，如NIH 3T3成纤维细胞、淋巴样EL4细胞和原代CD4(+) T淋巴细胞，而HTLV-1颗粒则不具有感染性。当位于MuLV包膜糖蛋白羧基末端的R肽被删除时，嵌合颗粒的感染性增加了10倍。被Delta R嵌合病毒感染的原代鼠CD4(+) T淋巴细胞释放出的颗粒能够将感染传播到其他未感染的鼠类淋巴细胞。这种具有Mo-MuLV包膜糖蛋白和HTLV-1复制特性的嵌合病毒，在小鼠模型中研究HTLV-1的发病机制方面应该会很有用。
