Chudek J, Wilhelm M, Bugert P, Herbers J, Kovacs G
Department of Urology, Ruprecht-Karls-University, Heidelberg, Germany.
Int J Cancer. 1997 Oct 9;73(2):225-9. doi: 10.1002/(sici)1097-0215(19971009)73:2<225::aid-ijc11>3.0.co;2-e.
Multiple hemi- and homozygous interstitial deletions at chromosome 3p have been found in different types of cancer, including non-papillary renal cell carcinoma (RCC). To determine the frequency and size of deletions, we have analyzed paired normal and tumor DNA obtained from short-term cultures of 104 non-papillary RCCs with 29 microsatellite markers covering the entire chromosome 3p region. Deletion mapping provided evidence for terminal deletion, with the most distal breakpoint between loci D3S1606 and D3S3666 in 94 cases, whereas constitutional heterozygosity was retained at all loci in 4 RCCs. In 6 cases, interstitial deletions were detected. Deletion mapping detected the smallest overlapping region between loci D3S3666 and D3S1560, which corresponds to an approx. 55 cM genetic distance.
在包括非乳头状肾细胞癌（RCC）在内的不同类型癌症中，已发现3号染色体短臂存在多个半合子和纯合子间质性缺失。为了确定缺失的频率和大小，我们分析了从104例非乳头状RCC短期培养物中获得的配对正常和肿瘤DNA，使用29个微卫星标记覆盖整个3号染色体短臂区域。缺失图谱提供了末端缺失的证据，94例病例中最远端的断点位于D3S1606和D3S3666位点之间，而4例RCC的所有位点均保留了体质杂合性。在6例病例中检测到间质性缺失。缺失图谱检测到D3S3666和D3S1560位点之间最小的重叠区域，这对应于约55 cM的遗传距离。
