Kovacs G, Wilkens L, Papp T, de Riese W
Institute of Pathology, Albert-Ludwigs-University, Freiburg, Federal Republic of Germany.
J Natl Cancer Inst. 1989 Apr 5;81(7):527-30. doi: 10.1093/jnci/81.7.527.
Recent studies have shown that the deletion of chromosome 3p or the loss of DNA sequences at 3p is generally associated with the development of renal cell carcinoma (RCC). However, chromosome analysis of some papillary RCCs suggested that this type of tumor differs genotypically from the most common nonpapillary RCCs. Therefore, by using cytogenetic and molecular genetic approaches, we examined human papillary and nonpapillary RCCs for the loss of heterozygosity or homozygosity at the short arm of chromosome 3. The constitutional heterozygosity for the DNF15S2 locus and for one allele of the c-erbA beta and the c-raf-1 proto-oncogenes was lost in nonpapillary RCCs, whereas both alleles were retained in each papillary RCC analyzed. We conclude that the loss of DNA sequences at the chromosome 3p region is a genomic change occurring consistently in nonpapillary RCCs, but never occurring in papillary RCCs.
近期研究表明，3号染色体短臂缺失或3p处DNA序列丢失通常与肾细胞癌（RCC）的发生相关。然而，一些乳头状肾细胞癌的染色体分析表明，这类肿瘤在基因型上与最常见的非乳头状肾细胞癌不同。因此，我们采用细胞遗传学和分子遗传学方法，检测了人乳头状和非乳头状肾细胞癌3号染色体短臂上杂合性缺失或纯合性缺失情况。在非乳头状肾细胞癌中，DNF15S2位点以及c-erbAβ和c-raf-1原癌基因的一个等位基因的构成性杂合性丢失，而在所分析的每例乳头状肾细胞癌中两个等位基因均得以保留。我们得出结论，3号染色体p区域的DNA序列丢失是一种在非乳头状肾细胞癌中始终出现而在乳头状肾细胞癌中从未出现的基因组改变。
