Gabbianelli R, Falcioni G, Santroni A M, Fiorini R, Coppa G V, Kantar A
Department of M.C.A. Biology, University of Camerino, Italy.
J Biolumin Chemilumin. 1997 Mar-Apr;12(2):79-85. doi: 10.1002/(SICI)1099-1271(199703/04)12:2<79::AID-BIO431>3.0.CO;2-O.
Erythrocytes from trout Salmo irideus are characterized by four different hemoglobin components (HbI, HbII, HbIII and HbIV), HbI and HbIV being predominant. In this study we describe the interaction between trout hemoglobin (HbI and HbIV) and H2O2 using a chemiluminescence assay. Our data show that the reaction of hemoglobins with H2O2 produces a time-limited and significant increase of chemiluminescence signal. The half-life of the decay of this chemiluminescence signal was characteristic for each type of hemoglobin used. These results indicate the formation of excited molecules related to the interaction between trout hemoglobin and H2O2.
虹鳟鱼（Salmo irideus）的红细胞具有四种不同的血红蛋白成分（HbI、HbII、HbIII和HbIV），其中HbI和HbIV占主导地位。在本研究中，我们使用化学发光分析法描述了虹鳟血红蛋白（HbI和HbIV）与H2O2之间的相互作用。我们的数据表明，血红蛋白与H2O2的反应会使化学发光信号产生有时间限制的显著增加。这种化学发光信号衰减的半衰期对于所使用的每种血红蛋白类型而言都是独特的。这些结果表明，虹鳟血红蛋白与H2O2相互作用会形成激发态分子。
