高静水压对牛嗜铬细胞中BK通道的影响。
Effect of high hydrostatic pressure on the BK channel in bovine chromaffin cells.
作者信息
Macdonald A G
机构信息
Department of Biomedical Sciences, University of Aberdeen, Scotland, UK.
出版信息
Biophys J. 1997 Oct;73(4):1866-73. doi: 10.1016/S0006-3495(97)78217-1.
Abstract
The activity of the BK channel of bovine chromaffin cells was studied at high hydrostatic pressure, using inside-out patches in symmetrical KCl solution, Ca2+-free and at V(H) = -60 to -40 mV. Pressure increased the probability of channels being open (900 atm increasing the probability 30-fold), and it increased the minimum number of channels apparent in the patches. The pressure activation of the channel was reversed on decompression. Channel conductance was unaffected. It was shown that pressure did not act by raising the temperature, or by affecting [Ca] or pH, or the order of the membrane bilayer, and it was concluded that pressure most likely acted directly on the channel proteins and/or their modulating reactions.
摘要
在高静水压条件下，使用对称KCl溶液、无Ca2+且膜电位V(H) = -60至-40 mV的内向外膜片，研究了牛嗜铬细胞BK通道的活性。压力增加了通道开放的概率（900个大气压使概率增加30倍），并增加了膜片中明显可见的通道最小数量。通道的压力激活在减压时逆转。通道电导不受影响。结果表明，压力并非通过升高温度、影响[Ca]或pH值或膜双层的有序性起作用，得出的结论是，压力最有可能直接作用于通道蛋白和/或其调节反应。
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