利用施加的张力将蛋白质从DNA上分离。
Driving proteins off DNA using applied tension.
作者信息
Marko J F, Siggia E D
机构信息
Department of Physics, The University of Illinois at Chicago, 60607-7059, USA.
出版信息
Biophys J. 1997 Oct;73(4):2173-8. doi: 10.1016/S0006-3495(97)78248-1.
Abstract
Proteins that bind DNA so as to reduce its end-to-end length can be dissociated by application of force. The thermodynamics of this process are discussed, with special attention to the case of histones bound to DNA (i.e., a string of nucleosomes, or chromatin fiber). The histone octamer is predicted to be driven off chromatin fiber for tensions >2 piconewtons.
摘要
能够结合DNA以缩短其端到端长度的蛋白质可通过施加力而解离。本文讨论了这一过程的热力学，特别关注了与DNA结合的组蛋白（即一串核小体或染色质纤维）的情况。预计当张力大于2皮牛顿时，组蛋白八聚体会从染色质纤维上被驱离。
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