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1
Transient gene expression from yeast artificial chromosome DNA in mammalian cells is enhanced by adenovirus.腺病毒可增强哺乳动物细胞中酵母人工染色体DNA的瞬时基因表达。
Nucleic Acids Res. 1997 Nov 1;25(21):4416-8. doi: 10.1093/nar/25.21.4416.
2
Analysis of a YAC with human telomeres and oriP from epstein-barr virus in yeast and 293 cells.对含有人类端粒和来自爱泼斯坦-巴尔病毒的oriP的酵母人工染色体（YAC）在酵母和293细胞中的分析。
Nucleic Acids Res. 1999 Sep 15;27(18):3736-44. doi: 10.1093/nar/27.18.3736.
3
Functional human CFTR produced by stable Chinese hamster ovary cell lines derived using yeast artificial chromosomes.使用酵母人工染色体衍生的稳定中国仓鼠卵巢细胞系产生的功能性人囊性纤维化跨膜传导调节因子。
Hum Mol Genet. 1997 Jan;6(1):59-68. doi: 10.1093/hmg/6.1.59.
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Delivery of bacterial artificial chromosomes into mammalian cells with psoralen-inactivated adenovirus carrier.利用补骨脂素灭活的腺病毒载体将细菌人工染色体导入哺乳动物细胞。
Nucleic Acids Res. 1997 May 15;25(10):1950-6. doi: 10.1093/nar/25.10.1950.
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pSURF-2, a modified BAC vector for selective YAC cloning and functional analysis.pSURF-2，一种用于选择性酵母人工染色体（YAC）克隆和功能分析的改良细菌人工染色体（BAC）载体。
Biotechniques. 1999 Jul;27(1):164-70, 172, 175. doi: 10.2144/99271rr05.
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A dicistronic construct allows easy detection of human CFTR expression from YAC DNA in human cells.双顺反子构建体能够轻松检测人细胞中源自酵母人工染色体（YAC）DNA的人囊性纤维化跨膜传导调节因子（CFTR）的表达。
Nucleic Acids Res. 1997 Oct 15;25(20):4167-8. doi: 10.1093/nar/25.20.4167.
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Aerosol and lobar administration of a recombinant adenovirus to individuals with cystic fibrosis. II. Transfection efficiency in airway epithelium.对囊性纤维化患者进行重组腺病毒的气雾剂和肺叶给药。II. 气道上皮中的转染效率。
Hum Gene Ther. 2001 Jul 20;12(11):1383-94. doi: 10.1089/104303401750298544.
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CFTR intron 1 increases luciferase expression driven by CFTR 5'-flanking DNA in a yeast artificial chromosome.囊性纤维化跨膜传导调节因子（CFTR）内含子1增强了酵母人工染色体中由CFTR 5'侧翼DNA驱动的荧光素酶表达。
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10
An improved method for routine preparation of intact artificial chromosome DNA (340-1000 kb) for transfection into human cells.一种用于常规制备完整人工染色体DNA（340 - 1000 kb）以转染入人细胞的改进方法。
Nucleic Acids Res. 1999 Apr 1;27(7):1762-5. doi: 10.1093/nar/27.7.1762.

本文引用的文献

1
Delivery of bacterial artificial chromosomes into mammalian cells with psoralen-inactivated adenovirus carrier.利用补骨脂素灭活的腺病毒载体将细菌人工染色体导入哺乳动物细胞。
Nucleic Acids Res. 1997 May 15;25(10):1950-6. doi: 10.1093/nar/25.10.1950.
2
Formation of de novo centromeres and construction of first-generation human artificial microchromosomes.从头合成着丝粒的形成及第一代人类人工微小染色体的构建。
Nat Genet. 1997 Apr;15(4):345-55. doi: 10.1038/ng0497-345.
3
Human artificial chromosomes get real.人类人工染色体成为现实。
Nat Genet. 1997 Apr;15(4):333-5. doi: 10.1038/ng0497-333.
4
Isolation of a common receptor for Coxsackie B viruses and adenoviruses 2 and 5.柯萨奇B病毒以及腺病毒2型和5型共同受体的分离
Science. 1997 Feb 28;275(5304):1320-3. doi: 10.1126/science.275.5304.1320.
5
Functional human CFTR produced by stable Chinese hamster ovary cell lines derived using yeast artificial chromosomes.使用酵母人工染色体衍生的稳定中国仓鼠卵巢细胞系产生的功能性人囊性纤维化跨膜传导调节因子。
Hum Mol Genet. 1997 Jan;6(1):59-68. doi: 10.1093/hmg/6.1.59.
6
Transgenic mice carrying an Xist-containing YAC.携带含Xist酵母人工染色体的转基因小鼠。
Hum Mol Genet. 1996 Apr;5(4):441-50. doi: 10.1093/hmg/5.4.441.
7
Adenovirus-mediated augmentation of cell transfection with unmodified plasmid vectors.腺病毒介导的未修饰质粒载体细胞转染增强作用。
J Biol Chem. 1993 Feb 5;268(4):2300-3.
8
Diversity of airway epithelial cell targets for in vivo recombinant adenovirus-mediated gene transfer.体内重组腺病毒介导的基因转移的气道上皮细胞靶标的多样性。
J Clin Invest. 1993 Jan;91(1):225-34. doi: 10.1172/JCI116175.
9
Microinjection of intact 200- to 500-kb fragments of YAC DNA into mammalian cells.将完整的200至500千碱基对酵母人工染色体（YAC）DNA片段显微注射到哺乳动物细胞中。
Genomics. 1993 Mar;15(3):659-67. doi: 10.1006/geno.1993.1121.
10
Stimulation of erythropoiesis by in vivo gene therapy: physiologic consequences of transfer of the human erythropoietin gene to experimental animals using an adenovirus vector.体内基因疗法对红细胞生成的刺激作用：使用腺病毒载体将人促红细胞生成素基因转移至实验动物的生理后果。
Blood. 1994 Nov 1;84(9):2946-53.

腺病毒可增强哺乳动物细胞中酵母人工染色体DNA的瞬时基因表达。

Transient gene expression from yeast artificial chromosome DNA in mammalian cells is enhanced by adenovirus.

作者信息

Chen M, Compton S T, Coviello V F, Green E D, Ashlock M A

机构信息

Vector Development Section, Genetics and Molecular Biology Branch and Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA.

出版信息

Nucleic Acids Res. 1997 Nov 1;25(21):4416-8. doi: 10.1093/nar/25.21.4416.

DOI:10.1093/nar/25.21.4416
PMID:9336477
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC147053/
Abstract

The introduction of high molecular weight DNA into mammalian cells is useful for gene expression studies. However, current transfection strategies are inefficient, necessitating propagation of stable DNA transformants prior to analysis of gene expression. Here we demonstrate that transient lipid-mediated DNA transfection can be used to assess gene expression from yeast artificial chromosomes (YACs) containing the 230 kb cystic fibrosis transmembrane conductance regulator gene ( CFTR ) and Escherichia coli lacZ . We also show that psoralen-UV inactivated adenovirus significantly enhances transfection efficiency. The ability to deliver high molecular weight DNA using lipid-mediated transfection should expedite the analysis of large human genes contained within artificial chromosome vectors.

摘要

将高分子量DNA导入哺乳动物细胞对基因表达研究很有用。然而，目前的转染策略效率低下，在分析基因表达之前需要稳定DNA转化体的增殖。在此我们证明，瞬时脂质介导的DNA转染可用于评估含有230 kb囊性纤维化跨膜传导调节基因（CFTR）和大肠杆菌lacZ的酵母人工染色体（YAC）的基因表达。我们还表明，补骨脂素-紫外线灭活的腺病毒可显著提高转染效率。利用脂质介导的转染递送高分子量DNA的能力应会加快对人工染色体载体中所含大型人类基因的分析。