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Multiple isoform recovery (MIR)-PCR: a simple method for the isolation of related mRNA isoforms.多亚型回收（MIR）-PCR：一种分离相关mRNA亚型的简单方法。
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本文引用的文献

1
Isolation of rare transcripts by representational difference analysis.通过代表性差异分析分离稀有转录本。
Nucleic Acids Res. 1997 Jul 1;25(13):2681-2. doi: 10.1093/nar/25.13.2681.
2
A novel PCR technique using Alu-specific primers to identify unknown flanking sequences from the human genome.一种使用铝特异性引物从人类基因组中鉴定未知侧翼序列的新型聚合酶链反应（PCR）技术。
Genomics. 1995 Sep 20;29(2):403-8. doi: 10.1006/geno.1995.9004.
3
Retroviral insertional mutagenesis as a strategy to identify cancer genes.逆转录病毒插入诱变作为一种识别癌症基因的策略。
Biochim Biophys Acta. 1996 May 16;1287(1):29-57. doi: 10.1016/0304-419x(95)00020-g.
4
Transient transfection of ecotropic retrovirus receptor permits stable gene transfer into non-rodent cells with murine retroviral vectors.嗜亲性逆转录病毒受体的瞬时转染可使鼠类逆转录病毒载体将基因稳定转入非啮齿类细胞。
Nucleic Acids Res. 1996 Mar 1;24(5):979-80. doi: 10.1093/nar/24.5.979.
5
Promoter region of mouse Tcrg genes.小鼠Tcrg基因的启动子区域
Immunogenetics. 1996;43(1-2):68-71. doi: 10.1007/BF00186606.
6
New human erg isoforms generated by alternative splicing are transcriptional activators.通过可变剪接产生的新的人erg异构体是转录激活因子。
Oncogene. 1993 Jul;8(7):1865-73.
7
An ets-related gene, ERG, is rearranged in human myeloid leukemia with t(16;21) chromosomal translocation.一个与ets相关的基因ERG，在伴有t(16;21)染色体易位的人类髓系白血病中发生重排。
Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10280-4. doi: 10.1073/pnas.90.21.10280.
8
Amplification and sequence analysis of DNA flanking integrated proviruses by a simple two-step polymerase chain reaction method.通过一种简单的两步聚合酶链反应方法对整合前病毒侧翼的DNA进行扩增和序列分析。
J Virol. 1993 Dec;67(12):7118-24. doi: 10.1128/JVI.67.12.7118-7124.1993.
9
Enhancement of retrovirus-mediated gene transduction efficiency by transient overexpression of the amphotropic receptor, GLVR-2.通过瞬时过表达双嗜性受体GLVR-2提高逆转录病毒介导的基因转导效率。
Nucleic Acids Res. 1995 Jun 11;23(11):2080-1. doi: 10.1093/nar/23.11.2080.
10
Excision of specific DNA-sequences from integrated retroviral vectors via site-specific recombination.通过位点特异性重组从整合的逆转录病毒载体中切除特定DNA序列。
Nucleic Acids Res. 1995 Nov 11;23(21):4451-6. doi: 10.1093/nar/23.21.4451.

一种基于快速逆转录聚合酶链反应的方法，用于分离逆转录病毒整合位点侧翼的互补DNA片段。

A rapid RT-PCR based method to isolate complementary DNA fragments flanking retrovirus integration sites.

作者信息

Valk P J, Joosten M, Vankan Y, Löwenberg B, Delwel R

机构信息

Institute of Hematology, Erasmus University Rotterdam, The Netherlands.

出版信息

Nucleic Acids Res. 1997 Nov 1;25(21):4419-21. doi: 10.1093/nar/25.21.4419.

DOI:10.1093/nar/25.21.4419
PMID:9336478
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC147027/
Abstract

Proto-oncogenes in retrovirally induced myeloid mouse leukemias are frequently activated following retroviral insertion. The identification of common virus integration sites (VISs) and isolation of the transforming oncogene is laborious and time consuming. We established a rapid and simple PCR based procedure which facilitates the identification of VISs and novel proto-oncogenes. Complementary DNA fragments adjacent to retrovirus integration sites were selectively isolated by applying a reverse transcriptase (RT) reaction using an oligo(dT)-adaptor primer, followed by PCR using the adaptor sequence and a retrovirus long terminal repeat (LTR) specific primer. Multiple chimeric cDNA fragments suitable for Southern and northern blot analysis were isolated.

摘要

逆转录病毒诱导的小鼠髓系白血病中的原癌基因在逆转录病毒插入后常常被激活。鉴定常见的病毒整合位点（VISs）并分离转化癌基因既费力又耗时。我们建立了一种基于PCR的快速简便方法，有助于鉴定VISs和新型原癌基因。通过使用寡聚（dT）-接头引物进行逆转录酶（RT）反应，选择性地分离与逆转录病毒整合位点相邻的互补DNA片段，随后使用接头序列和逆转录病毒长末端重复序列（LTR）特异性引物进行PCR。分离出了多个适用于Southern和Northern印迹分析的嵌合cDNA片段。