Zhang Z, Wang Y, Ruan J
Microbial Collection and Screening Laboratory, National University of Singapore, Singapore.
Int J Syst Bacteriol. 1997 Oct;47(4):1048-54. doi: 10.1099/00207713-47-4-1048.
We determined almost complete 16S ribosomal DNA sequences for 12 actinomycete strains which were either previously classified as Kitasatospora strains or defined as Streptomyces strains but shown to contain major amounts of meso-diaminopimelic acid in their whole-cell hydrolysates. These sequences were subjected to phylogenetic analyses together with the sequences of 34 Streptomyces species. Phylogenetic trees were reconstructed by using both neighbor-joining and maximum-parsimony methods. The Kitasatospora species always formed a stable monophyletic clade. However, the genus Kitasatospora appeared to be either a sister taxon of the genus Streptomyces or a lineage that originated from within Streptomyces species, depending on the outgroup used. Phylogenetic trees were also constructed by using the sequences of the 16S-23S rRNA gene spacers. Streptomyces and Kitasatospora species were consistently recovered as two distinct clades independent of the outgroup used. On the basis of phylogenetic, chemotaxonomic, and phenotypic evidence, we propose that the genus Kitasatospora Omura et al. 1982 should be revived.
我们测定了12株放线菌菌株几乎完整的16S核糖体DNA序列，这些菌株要么先前被归类为北里孢菌属菌株，要么被定义为链霉菌属菌株，但在其全细胞水解产物中显示含有大量内消旋二氨基庚二酸。将这些序列与34种链霉菌属物种的序列一起进行系统发育分析。使用邻接法和最大简约法重建系统发育树。北里孢菌属物种总是形成一个稳定的单系分支。然而，根据所使用的外类群，北里孢菌属似乎要么是链霉菌属的姐妹分类单元，要么是起源于链霉菌属物种内部的一个谱系。还使用16S - 23S rRNA基因间隔区序列构建了系统发育树。链霉菌属和北里孢菌属物种始终被归为两个不同的分支，与所使用的外类群无关。基于系统发育、化学分类和表型证据，我们提议恢复1982年大村等人建立的北里孢菌属。
