Chyczewska E, Mróz R M, Kowal E
Department of Pneumonology and Tuberculosis, Medical Academy of Białystok.
Rocz Akad Med Bialymst. 1997;42 Suppl 1:123-35.
Fiberoptic bronchoscopy and bronchoalveolar lavage (BAL) provide facilities for biologically active substances directly produced by the tumor. In the present study we have investigated the concentration of the following cytokines: TNF-alpha, IL-2 and IL-6 in bronchoalveolar lavage fluid (BALF) of non-small cell lung cancer (NSCLC) before treatment. The study group consisted of 20 patients with squamous cell carcinoma (Gr. I). The control group consisted of 18 patients with non-malignant lung disease (6 patients with sarcoidosis and 12 with COPD). All patients underwent bronchoscopy followed by bronchoalveolar lavage (BAL). The concentrations of the above mentioned cytokines were measured using Sorin's ETI system for EIA analysis. Statistical analysis was performed within the groups, between the groups, and in different stages of malignant disease. The mean TNF-alpha concentration in Gr. I was 1192 pg/ml/mg p. and was significantly higher than in sarcoidosis (5.3 pg/ml/ mg p.) and COPD (0.5 pg/ml/mg p.). We observed a correlation between TNF-alpha concentration and the stage of malignant disease. The highest concentration was in IIIb stage (2150 pg/ml/mg p.). IL-6 concentration in malignant patients was strongly correlated with TNF-alpha concentration and was significantly higher than in control (265.868 pg/ml/mg p. in cancer patients, in sarcoidosis: 21.694 pg/ml/mg p. and in COPD: 40.708 pg/ml/mg p.). It was the highest in stage II (334 pg/ml/mg p.). IL-1 concentrations were not significantly higher in malignant patients (50.173 pg/ml/mg p., nor in IIIa stage (70.136) pg/ml/mg p. as compared with controls (18.648 pg/ml/mg p. in sarcoidosis and 28.395 pg/ml/mg p. in COPD).
纤维支气管镜检查和支气管肺泡灌洗（BAL）为肿瘤直接产生的生物活性物质提供了检测手段。在本研究中，我们调查了非小细胞肺癌（NSCLC）患者治疗前支气管肺泡灌洗液（BALF）中以下细胞因子的浓度：肿瘤坏死因子-α（TNF-α）、白细胞介素-2（IL-2）和白细胞介素-6（IL-6）。研究组由20例鳞状细胞癌患者（I级）组成。对照组由18例非恶性肺部疾病患者组成（6例结节病患者和12例慢性阻塞性肺疾病（COPD）患者）。所有患者均接受了支气管镜检查及随后的支气管肺泡灌洗（BAL）。使用索林公司的ETI系统进行酶免疫分析来测定上述细胞因子的浓度。在组内、组间以及恶性疾病的不同阶段进行了统计分析。I级患者中TNF-α的平均浓度为1192 pg/ml/mg蛋白，显著高于结节病患者（5.3 pg/ml/ mg蛋白）和COPD患者（0.5 pg/ml/mg蛋白）。我们观察到TNF-α浓度与恶性疾病分期之间存在相关性。最高浓度出现在IIIb期（2150 pg/ml/mg蛋白）。恶性患者中IL-6的浓度与TNF-α浓度密切相关，且显著高于对照组（癌症患者为265.868 pg/ml/mg蛋白，结节病患者为21.694 pg/ml/mg蛋白，COPD患者为40.708 pg/ml/mg蛋白）。在II期最高（334 pg/ml/mg蛋白）。恶性患者中IL-1的浓度与对照组相比没有显著升高（恶性患者为50.173 pg/ml/mg蛋白，IIIa期为70.136 pg/ml/mg蛋白，而结节病对照组为18.648 pg/ml/mg蛋白，COPD对照组为28.395 pg/ml/mg蛋白）。
