Loginova N V, Trotsenko I U
Mikrobiologiia. 1976 Mar-Apr;45(2):217-23.
The oxidation by Arthrobacter globiformis B-175 of tertiary, secondary, and primary amines is catalyzed by specific induced enzymes. Trimethylamine is oxidized to dimethylamine via trimethylamine N-oxide by monooxygenase and demethylase. Dimethylamine is converted to methylamine by another monooxygenase. Methylamine is oxidized to ammonia and formaldehyde by aminoxidase. Formaldehyde is assimilated via ribulose monophosphate cycle, which is confirmed by the presence of hexose-phosphate synthase and by the early and predominant formation of radioactive sugar phosphates during short-term incubation of the cells with labelled methylated amines. Hexose-phosphate synthase was also found in two other strains of A. globiforms (B-126, B-53) which assimilated methylated amines. This methylotroph contains the enzymes of the Krebs cycle which seems to be involved in the synthesis of organic acids. The participation of ammonium nitrogen in anabolic processes is accomplished by reductive amination of alpha-ketoglutarate, glyoxylate, and pyruvate.
球形节杆菌B - 175对叔胺、仲胺和伯胺的氧化作用由特定的诱导酶催化。三甲胺通过单加氧酶和脱甲基酶经三甲胺N - 氧化物氧化为二甲胺。二甲胺由另一种单加氧酶转化为甲胺。甲胺通过氨基氧化酶氧化为氨和甲醛。甲醛通过磷酸戊糖循环被同化，这通过磷酸己糖合酶的存在以及在细胞与标记甲基化胺短期孵育期间放射性糖磷酸的早期和主要形成得到证实。在另外两株同化甲基化胺的球形节杆菌（B - 126、B - 53）中也发现了磷酸己糖合酶。这种甲基营养菌含有似乎参与有机酸合成的三羧酸循环的酶。铵态氮在合成代谢过程中的参与是通过α - 酮戊二酸、乙醛酸和丙酮酸的还原胺化作用来实现的。
