Tiunova N A, Pirieva D A, Feniksova R V, Kuznetsov V D
Mikrobiologiia. 1976 Mar-Apr;45(2):280-3.
Actinomycetes were cultivated in the medium containing demineralized crab shells as a source of carbon. Chitinase was found in filtrates of the cultural broth of all 44 studied strains. The activity of the extracellular chitinase was determined both by decomposition of ground chitin (CHI-activity) and colloid chitin (CHI-activity). The ratio between CHI and CHX in filtrates of the cultural broth of the most active nine strains varied from one to two.
放线菌在含有脱矿质蟹壳作为碳源的培养基中培养。在所研究的44株菌株的培养液滤液中均发现了几丁质酶。通过对磨碎的几丁质(CHI活性)和胶体几丁质(CHI活性)的分解来测定细胞外几丁质酶的活性。活性最高的9株菌株的培养液滤液中CHI与CHX的比例在1到2之间变化。