Plummer S J, Simmons J A, Adams L, Casey G
Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, Ohio 44195, USA.
Genomics. 1997 Oct 1;45(1):140-6. doi: 10.1006/geno.1997.4906.
We have integrated genetic and physical mapping data for chromosome 17 subdivided into 26 bins, by using a panel of chromosome 17 deletion somatic cell hybrids. One hundred four short tandem repeat and STS markers have been localized into these bins and have enabled the ordering of 288 ESTs and 26 genes, including 142 ESTs that had not been previously sublocalized on chromosome 17. The mapping information of several genetic maps, as well as information obtained by radiation hybrid and STS content mapping of YACs, has been integrated using this hybrid panel. Although existing mapping information for chromosome 17 was generally consistent for many ESTs previously mapped, the map presented here further refines the location of ESTs, as well as demonstrating a number of discrepancies found in the 17q24-q25 region. We attribute these discrepancies to the fact that the current radiation hybrid panels were selected for retention of the thymidine kinase gene at 17q25, as well as to a low concentration of YAC contigs in this region. These data illustrate the benefit of combining multiple mapping techniques to obtain the greatest accuracy. The integration of maps developed by different methods will generate the most accurate genome maps, which may then be used for the generation of large insert clone contigs for chromosome sequencing. Additionally, accurate transcript maps generated by ESTs will greatly speed the isolation of genes linked to disease loci.
我们利用一组17号染色体缺失体细胞杂种，整合了17号染色体细分为26个区间的遗传和物理图谱数据。104个短串联重复序列和STS标记已定位到这些区间，并实现了288个EST和26个基因的排序，其中包括142个以前未在17号染色体上进行亚定位的EST。利用该杂种细胞系整合了几个遗传图谱的定位信息，以及通过YACs的辐射杂种和STS含量图谱获得的信息。尽管17号染色体现有的定位信息对于许多先前定位的EST总体上是一致的，但本文给出的图谱进一步细化了EST的位置，同时也显示了在17q24-q25区域发现的一些差异。我们将这些差异归因于当前的辐射杂种细胞系是为保留17q25处的胸苷激酶基因而选择的，以及该区域YAC重叠群的浓度较低。这些数据说明了结合多种定位技术以获得最高准确性的好处。通过不同方法构建的图谱整合将产生最准确的基因组图谱，然后可用于生成用于染色体测序的大插入片段克隆重叠群。此外，由EST生成的准确转录图谱将极大地加速与疾病基因座连锁的基因的分离。
