Kinoshita-Iramina C, Kitahara M, Doi K, Ogata S
Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Biosci Biotechnol Biochem. 1997 Sep;61(9):1469-73. doi: 10.1271/bbb.61.1469.
Plasmid pSLL of Streptomyces laurentii ATCC31255 (wild-type strain P0) is a 93-kilobase linear DNA plasmid that carries a protein bound to each 5' end of the DNA. It was self-transmitted to the pSLL-cured strain by conjugation in solid culture. The pSLL-cured strain carried a circular plasmid, pSLS, and showed a marked decrease in spore formation and thiostrepton productivity, owing to the pSLS. However, by retransmission of pSLL, these things reverted to levels seen in strain P0. Thus, plasmid pSLL suppressed the injurious effects of pSLS on the host mycelia.
劳伦链霉菌ATCC31255（野生型菌株P0）的质粒pSLL是一种93千碱基的线性DNA质粒，其DNA的每个5'端都结合有一个蛋白质。它在固体培养中通过接合作用自我传递给pSLL缺失菌株。pSLL缺失菌株携带一个环状质粒pSLS，由于pSLS的存在，其孢子形成和硫链丝菌素产量显著下降。然而，通过pSLL的重新传递，这些情况恢复到了菌株P0中的水平。因此，质粒pSLL抑制了pSLS对宿主菌丝体的有害影响。
