Ito Y, Jono H, Shojo H
Department of Forensic Medicine, Saga Medical School, Japan.
Kurume Med J. 1997;44(3):209-15. doi: 10.2739/kurumemedj.44.209.
The effects of chronic administration of methamphetamine on pancreatic tissues were histopathologically studied in experimental models. Methamphetamine (1 ml/kg body weight/day) was subcutaneously injected into 14 five-week-old male Wistar Kyoto rats (WKY) for 12 weeks. Age and sex matched 5 WKY rats served as controls. With light microscopy, some scattered edematous lesions and moderate vacuolization were demonstrated in the pancreas of 8 of the methamphetamine treated rats. However, in 4 of the rats, severe regional hemorrhage, partial acinal cell necrosis, destruction of the acinal cells, neutrophile infiltration, interstitial vessel dilatation, interstitial edema and fatty cell invasion were observed after the injections of methamphetamine. In 2 other animals, fibrosis and cirrhosis-like lesions with destruction and degeneration of the acinal cells were observed the small vessels had a slight degeneration of the endothelial cells. In the control animals, no lesions, except for some edematous lesions were found. In all cases, there were no nesidioblastosis-like lesions or necrosis of the Langerhans's islets. In the immunohistochemical study using anti- alpha 1-chymotrypsin antibody, more positive reactive cells were demonstrated among the interstitial and inter acinal cells, both in number and degree, in the methamphetamine treated rats. In addition to the animal model, there were 4 autopsy cases of sudden death in chronic methamphetamine abusers. The autopsies demonstrated a severe acute necrotic hemorrhagic pancreatitis, with only scattered slight hemorrhaging in the brain and lungs. These findings indicate that chronic administration of methamphetamine to rats evoked significant changes in pancreatic tissues including some degeneration of the endothelial cells of the small vessels in this hypoxia-vulnerable organ.
在实验模型中对长期给予甲基苯丙胺对胰腺组织的影响进行了组织病理学研究。将甲基苯丙胺（1毫升/千克体重/天）皮下注射到14只5周龄雄性Wistar Kyoto大鼠（WKY）体内，持续12周。5只年龄和性别匹配的WKY大鼠作为对照。通过光学显微镜观察，在8只接受甲基苯丙胺治疗的大鼠胰腺中发现了一些散在的水肿性病变和中度空泡化。然而，在4只大鼠中，注射甲基苯丙胺后观察到严重的局部出血、部分腺泡细胞坏死、腺泡细胞破坏、中性粒细胞浸润、间质血管扩张、间质水肿和脂肪细胞浸润。在另外2只动物中，观察到纤维化和肝硬化样病变，伴有腺泡细胞的破坏和变性，小血管内皮细胞有轻微变性。在对照动物中，除了一些水肿性病变外未发现其他病变。在所有病例中，均未发现胰岛母细胞增生样病变或朗格汉斯胰岛坏死。在使用抗α1 - 糜蛋白酶抗体的免疫组织化学研究中，在接受甲基苯丙胺治疗的大鼠的间质和腺泡间细胞中，阳性反应细胞在数量和程度上均更多。除动物模型外，有4例慢性甲基苯丙胺滥用者猝死的尸检病例。尸检显示为严重的急性坏死性出血性胰腺炎，仅在脑和肺中有散在的轻微出血。这些发现表明，对大鼠长期给予甲基苯丙胺会引起胰腺组织的显著变化，包括这个对缺氧敏感器官中小血管内皮细胞的一些变性。
