Mergia A, Blackwell J, Chari S
Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville 32610-0145, USA.
AIDS Res Hum Retroviruses. 1997 Oct 10;13(15):1333-9. doi: 10.1089/aid.1997.13.1333.
The feline immunodeficiency virus (FIV) Rev protein and its cognate sequence the Rev response element (RRE) are essential for virus replication. Thus, the inhibition of either Rev or RRE function can significantly inhibit FIV replication. In the present study, we constructed a ribozyme that targets the RRE sequence and determined its ability to inhibit FIV replication. The RRE ribozyme cleaved the target molecule both in vitro and in FIV-infected cells. Furthermore, FIV replication was reduced significantly in the presence of the RRE ribozyme. FIV provides a good animal model system with which to develop novel antiviral strategies for the human immunodeficiency virus.
猫免疫缺陷病毒（FIV）的Rev蛋白及其同源序列Rev反应元件（RRE）对病毒复制至关重要。因此，抑制Rev或RRE的功能均可显著抑制FIV复制。在本研究中，我们构建了一种靶向RRE序列的核酶，并测定了其抑制FIV复制的能力。RRE核酶在体外和FIV感染的细胞中均能切割靶分子。此外，在RRE核酶存在的情况下，FIV复制显著减少。FIV为开发针对人类免疫缺陷病毒的新型抗病毒策略提供了一个良好的动物模型系统。
