Kang S J, Kim K S, Park K S
Department of Science Education, Ewha Womans University, Seoul, Korea.
Mol Cells. 1997 Aug 31;7(4):482-8.
The alcohol dehydrogenase (ADH) activity and ADH cross reacting material (ADH CRM) were measured and Northern blot analysis was carried to define the function and the regulation mechanism of the Adh gene. This study examined how dietary threonine affects the expression of the Adh gene during development of Drosophila melanogaster. Two wild type strains, one homozygous for Adh(F) and one for Adh(S) from Chunan, Korea were used. The ADH activity and CRMs of the Adh(F) strain were 2.1 times higher than those of Adhs strain, and ADH activity was higher with isopropanol (secondary alcohol) than with ethanol (primary alcohol) as a substrate in both Adh(F) and Adh(S) strains. When the larvae, pupae, newly emerged adults (0-1 day), and adults (5-7 days) of Drosophila melanogaster Adh(F) and Adh(S) strains were fed on a defined low yeast and threonine medium, ADH activity and ADH CRM levels were increased. Northern blot analyses indicated that the production of mRNA of the larvae, young adults (0-1 day), and adults (5-7 days) was increased by dietary threonine. ADH activity and ADH CRM increases in Drosophila melanogaster fed on threonine were as a result of threonine-stimulated alteration in the amount of ADH mRNA. The elevated level of the ADH mRNA transcribed from the proximal and distal promoters of threonine-fed larvae and adults showed that there was an induction.
测定了乙醇脱氢酶（ADH）活性和ADH交叉反应物质（ADH CRM），并进行了Northern印迹分析以确定Adh基因的功能和调控机制。本研究考察了日粮苏氨酸如何影响黑腹果蝇发育过程中Adh基因的表达。使用了两个野生型品系，一个是Adh(F)纯合子，另一个是来自韩国春川的Adh(S)纯合子。Adh(F)品系的ADH活性和CRMs比Adh(S)品系高2.1倍，并且在Adh(F)和Adh(S)品系中，以异丙醇（仲醇）为底物时的ADH活性高于以乙醇（伯醇）为底物时的活性。当用限定的低酵母和苏氨酸培养基喂养黑腹果蝇Adh(F)和Adh(S)品系的幼虫、蛹、新羽化的成虫（0 - 1天）和成蝇（5 - 7天）时，ADH活性和ADH CRM水平升高。Northern印迹分析表明，日粮苏氨酸可增加幼虫、年轻成虫（0 - 1天）和成蝇（5 - 7天）mRNA的产生。在以苏氨酸为食的黑腹果蝇中，ADH活性和ADH CRM的增加是苏氨酸刺激ADH mRNA量改变的结果。从苏氨酸喂养的幼虫和成虫的近端和远端启动子转录的ADH mRNA水平升高表明存在诱导作用。
