Burger J, Hummel S, Herrmann B
Institut für Anthropologie, Universität Göttingen.
Anthropol Anz. 1997 Jun;55(2):193-8.
DNA-extracts from prehistoric roots of teeth were analyzed by PCR to investigate microsatellite-systems. At the same time the x/y-chromosome specific system Amel A/B was investigated. The samples were collected from different burial conditions of similar age. Factors leading to limited DNA-degradation are given. For the first time reproducible Quadruplex-amplification on single-copy loci of prehistoric tissue was obtained in samples from the Lichtenstein-Cave, Kr. Osterode/Harz. Positive results are explained by low temperature in the burial site. In addition the results show that desiccation allows DNA-preservation but cannot protect the DNA from damage due to microbial origin. Microorganisms can destroy DNA-structures completely.
通过聚合酶链式反应（PCR）分析史前牙齿根部的DNA提取物，以研究微卫星系统。同时，对X/Y染色体特异性系统Amel A/B进行了研究。样本采集自年龄相近但埋葬条件不同的情况。文中给出了导致DNA降解受限的因素。首次在来自下萨克森州奥斯特罗德县/哈茨地区利希滕斯坦洞穴的样本中，实现了对史前组织单拷贝基因座的可重复四重扩增。阳性结果归因于埋葬地点的低温。此外，结果表明干燥有利于DNA保存，但无法保护DNA免受微生物来源的损害。微生物能够完全破坏DNA结构。
