在阿尔茨海默病大脑中，活化的小胶质细胞与β-淀粉样蛋白的血管周围沉积物共定位。
Activated microglial cells are colocalized with perivascular deposits of amyloid-beta protein in Alzheimer's disease brain.
作者信息
Uchihara T, Akiyama H, Kondo H, Ikeda K
机构信息
Department of Neurology, Tokyo Medical and Dental University, Japan.
出版信息
Stroke. 1997 Oct;28(10):1948-50. doi: 10.1161/01.str.28.10.1948.
BACKGROUND AND PURPOSE
Microglial cells are present in the center of senile plaques (SPs) in Alzheimer's disease (AD) brain. Such a localization of microglial cells suggests that they are involved in the deposition or the clearance of amyloid-beta protein (A beta) in the brain. We examined their association with another type of parenchymal A beta deposit, which is termed the perivascular deposits of A beta (PA beta).
METHODS
Thick sections from AD brain were stained with a three-color immunofluorescence method that labeled A beta, activated microglial cells, and vascular endothelial cells simultaneously.
RESULTS
Three-dimensional observation under a laser scanning microscope confirmed that perivascular aggregates of activated microglial cells were colocalized with PA beta.
CONCLUSIONS
Microglia occur in association with both SPs and PA beta, suggesting that they play important roles in the metabolism of A beta in AD brain.
背景与目的
在阿尔茨海默病（AD）脑内，小胶质细胞存在于老年斑（SPs）的中心。小胶质细胞的这种定位表明它们参与了脑内β淀粉样蛋白（Aβ）的沉积或清除。我们研究了它们与另一种实质内Aβ沉积物的关系，这种沉积物被称为β淀粉样蛋白的血管周围沉积物（PAβ）。
方法
采用三色免疫荧光法对AD脑的厚切片进行染色，同时标记Aβ、活化的小胶质细胞和血管内皮细胞。
结果
激光扫描显微镜下的三维观察证实，活化的小胶质细胞的血管周围聚集物与PAβ共定位。
结论
小胶质细胞与SPs和PAβ均有关联，提示它们在AD脑内Aβ的代谢中起重要作用。
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