Moresco R M, Scheithauer B W, Lucignani G, Lombardi D, Rocca A, Losa M, Casati R, Giovanelli M, Fazio F
INB-CNR, Department of Nuclear Medicine, University of Milan, H.S. Raffaele, Italy.
Nucl Med Commun. 1997 Jul;18(7):606-15. doi: 10.1097/00006231-199707000-00003.
There have been a number of indications that sex hormones can affect the rate of growth of meningiomas during pregnancy. The presence of oestrogen or progesterone receptors in meningiomas and the influence of sex hormones upon cell cultures derived from human meningiomas have been extensively investigated. The results have been controversial, with most of the discussion centring upon the presence and possible role of oestrogen receptors. The aim of the present study was to assess oestrogen receptors in human meningiomas with 16alpha[l8F]fluoro-17beta-oestradiol ([18F]FES) and positron emission tomography (PET). With this purpose in mind, we measured the regional brain uptake of [18F]FES in six patients with a neuroradiological and histological diagnosis of meningioma, comparing the in vivo PET data with the immunohistological analysis of oestrogen receptors performed on formalin-fixed, paraffin-embedded tissue obtained at surgery. Two analyses were used for the in vivo measurement of [18F]FES binding to oestrogen receptors: the ratio of tumour activity to that of normal tissue (T/NT), calculated 90 min after tracer injection, and the ratio between the equilibrium distribution volume (DV) in normal and pathological tissues, calculated by means of a graphical kinetic analysis. PET studies demonstrated a marked uptake of [18F]FES by the tumour in four of the six patients. Immunohistochemical assay using a manual staining method capable of detecting oestrogen receptors at a level of > 10 pmol mg(-1) of protein detected only sparse immunostaining in one of the six meningiomas. Distinct albeit weak immunostaining was demonstrated in five of the six meningiomas when the sensitivity of the immunohistochemical assay was increased to < 10 pmol mg(-1) of protein by use of an automated staining method (Bioteck 1000). Comparison of the in vivo and immunohistochemical results showed a correlation in five of the six patients, thus indicating the high sensitivity of [18F]FES for the in vivo evaluation of oestrogen receptor expression.
已有多项迹象表明，性激素可影响孕期脑膜瘤的生长速度。脑膜瘤中雌激素或孕激素受体的存在以及性激素对源自人类脑膜瘤的细胞培养物的影响已得到广泛研究。结果存在争议，大多数讨论集中在雌激素受体的存在及其可能的作用上。本研究的目的是使用16α[18F]氟-17β-雌二醇（[18F]FES）和正电子发射断层扫描（PET）评估人类脑膜瘤中的雌激素受体。出于这个目的，我们测量了6例经神经放射学和组织学诊断为脑膜瘤的患者大脑中[18F]FES的区域摄取情况，将体内PET数据与对手术获取的福尔马林固定、石蜡包埋组织进行的雌激素受体免疫组织学分析进行比较。采用两种分析方法对[18F]FES与雌激素受体的结合进行体内测量：示踪剂注射90分钟后计算肿瘤活性与正常组织活性的比值（T/NT），以及通过图形动力学分析计算正常组织和病理组织中平衡分布容积（DV）的比值。PET研究显示，6例患者中有4例肿瘤对[18F]FES有明显摄取。使用能够检测蛋白水平>10 pmol mg(-1)的雌激素受体的手工染色方法进行免疫组织化学检测时，6例脑膜瘤中只有1例显示稀疏免疫染色。当使用自动染色方法（Bioteck 1000）将免疫组织化学检测的灵敏度提高到<10 pmol mg(-1)的蛋白时，6例脑膜瘤中有5例显示出明显但较弱的免疫染色。体内和免疫组织化学结果的比较显示，6例患者中有5例存在相关性，从而表明[18F]FES对雌激素受体表达的体内评估具有高灵敏度。
